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Oxycycler GT4181C

CO Subchamber Cell Culture System

  • First CO Gasotransmitter Cell Culture System
  • Precise Automated Exposures of CO
  • Dynamic or Static Exposures
  • Simulate Physiologic and Pathophysiologic conditions
  • Unprecedented CO Induced Phenotypes
When you publish your data generated with this equipment, please copy and paste as follows for proper citation in your manuscript.

OxyCycler GT4181C Overview

ADVANCED GASOTRANSMITTER INCUBATION

Carbon monoxide (CO) is now known to be an exciting gasotransmitter involved in cell signaling, with powerful and interesting effects on cell phenotype. Until now, CO was difficult to study in cell culture because the only available method for applying CO in vitro used to be chemical donors. This method made it messy and difficult to control, and CO exposures were hard to reproduce. Now, with the OxyCycler GT4181C controller, cell culture in subchamber culture systems makes CO research easy, precise and very reproducible.

UNLIMITED PROGRAMMED, DYNAMIC EXPOSURES

Consisting of a GT41 which provides the common O2, CO2, and RH control, and the modular GT81C piggyback controller, which provides the CO, a subchamber cell culture system controlled by the OxyCycler GT4181C is the most advanced and reliable method for CO culture. Any dose and duration to cells in culture can be simply programmed. With the click of a mouse, any exposure can be precisely reproduced. Inside the culture chamber, all critical conditions are controlled by the OxyCycler GT4181C. Interface between the cells in culture and the GT4181C is completely protected by a microbial barrier. In addition, none of the sensors are directly exposed to the cells. This way, if the culture chamber gets contaminated, the sensors will not. Likewise, if the sensors ever get contaminated, they cannot contaminate your cells.

MODULAR FLEXIBILITY

The controller is compatible and will fit any standard size C-Chamber. The entire OxyCycle GT4181C cell culture system will fit any existing incubator.

  cell culture system will fit any existing incubator.

 

SYSTEM SPECIFICATION

ELECTRICAL POWER: 12VDC at 6.66a (power supply specifications), expected current draw around 3.7a.

CONTROL RANGE: O2: 0.1-99.9%, CO2: 0.1-20.0%, CO: 0-400PPM

ACCURACY: O2: ±1% at constant temperature/pressure, ±2% over entire temperature range. CO2: ±5% of measurement or 0.1% CO2. Temperature: ±0.6°C. Relative Humidity: ±3% RH between 0-40°C. CO: varies based on calibration for customer protocol

OXYGEN SENSOR: Electro-galvanic fuel cell

CARBON DIOXIDE SENSOR: Infrared sensing

CARBON MONOXIDE SENSOR: Electro-galvanic fuel cell

GAS SOURCE: Compressed gas tanks, liquid carboys (from headspace) or generators

GAS SUPPLY: Pressurized O2, CO2, N2, O2/CO2 SPAN mix, CO, CO SPAN mix. Customer should consider protocol when ordering SPAN gases. Appropriate CO volume and concentration to be determined by lab safety officer.

GAS SUPPLY LINE PRESSURE: 0-25 PSIG

GAS CONSUMPTION: Depends on (1) size and leakiness of host chamber, (2) frequency and duration of opening chamber doors and (3) gas level

GAS SUPPLY HOSE FITTING: 1/4” ID

UMBILICAL LENGTH: 12 ft

UMBILICAL DIAMETER: 1/16” ID

SENSOR CABLE LENGTH: 12 ft

SENSOR CABLE DIAMETER: 6mm

ALARM OUTPUT: Global Alarm Output and Audible, External Alarm system

ALARM MODES: Process High, Process Low, Deviation High, Deviation Low, Deviation Band

WEIGHT: 22 Lbs

CONTROLLER DIMENSIONS: 9”H, 17”W, 15”D

REMOTE MONITOR POD DIMENSIONS: 4.25”H, 7.0625”W, 4.25”D

SYSTEM SPECIFICATION

HOST CHAMBER TEMPERATURE:5-40°C HOST CHAMBER HUMIDITY:15-90%, Non-Condensing

GT81C

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GT41

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1. Bleed Valves and Barbs: Bleeds gases out of gas supply lines. Calibration cup for sensor attaches here.

2. Controller: Bright blue digits on black back ground. Continuously displays current control gas level, control status, and alarm status in all chambers. Displays menu items and settings during programming.

3. Alarm: Will sound if Ambient CO monitor detects unsafe levels.

4. ZERO Calibration Gas Flowmeter: Used for calibration.

5. SPAN Calibration Gas Flowmeter: Used for calibration.

6. Needle Valves: Sets infusion rate of control gases in each chamber to accommodate different dynamics. Can manually override controller to shut off gas.

7. Accessory Receptacle: 10 Pin Receptacle is for connecting optional accessory units.

8. Communications Cable Jack: This cable relays information for the sensors.

9. Actuator Pod Umbilical: Flexible umbilicals connect remote actuator pods to back panel.

10. Alarm Receptacle: Connect an appropriate alarm to this jack.

11. RS 485 Connections: One cable attaches to a computer and the other cable attaches to another unit, to allow communication with the computer (if applicable).

12. Pump Connection: This 3 pin receptacle supplies power to the Mini Pod Pump.

13. Supply Gas Hose Barb: Barbs for 1/4 inch I.D. hose from gas sources. Handles pressure up to 40 PSIG.

14. Span Mix Barb: Barb for 1/4 inch I.D. hose from gas source.

15. Ground Stud: For grounding the unit to protect from electric damage.

16. Power Receptacle: 12VDC power supply connects here.

17. Monitor Pod Umbilical: Flexible umbilical connects remote monitor pod to back panel. Semi-swivel connectors at both ends allow 360° orientation. Some models are hard welded; function is the same.

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Installation Schematic

OxyCycler GT4181C Culture System. Controls dynamic or static O2, CO2, and CO, and limits RH in subchamber. Supply gases to GT41 required: Oxygen, Nitrogen, Carbon Dioxide. Calibration gas to GT41 required: certified pre-mix of 10% CO2 in balance oxygen. Supply gases to GT81C: non-certified pre-mix of 10000ppm CO in balance nitrogen. Calibration gas to GT81C required: certified pre-mix of 300ppm CO in balance nitrogen. oxycyclergt4181c05

Configuration

The OxyCycler GT4181C is a combination controller consisting of a GT41 mother controller and the GT81C piggyback controller working in tandem. It makes CO gasotransmitter research easy and reproducible. The GT41 controls core O2 and CO2 levels in dynamic or static states and limits RH, while in conjunction the GT81C controls 0-400 ppm CO in dynamic or static exposures. Optionally, the GT81C can be removed and substituted with the GT81N for NO control with NO2 limiting, or the GT81CN for simultaneous CO and NO control with NO2 limiting.

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How It Works

Hot swap multipod adapter plate provides microbial barrier filters between cells culturing in the subchamber and all of the sensors and mechanisms that control O2, CO2, RH, CO and temperature. Hot swap multipods provide upgrade flexibility and immediate, easy maintenance. A pump draws a sample from the controlled atmosphere and passes by all sensors and returns to chamber through disposable microbial barrier filters. Appropriate gases are infused as necessary by controllers through terminal, microbial barrier filters. Small fan homogenizes gases throughout chamber and can be easily removed, sterilized and replaced. A loop pod (depicted to the far right) keeps a continuous flow of the sample draw in the absence of the NO/NO2 multipod. If the system is upgraded at a later date, the loop pod is easily removed and replaced with a NO/NO2 multipod. oxycyclergt4181c06Controller sits outside the incubator and umbilicals extend through port hole on the incubator and connect to Hot Swap Minipod Assembly inside the incubator. oxycyclergt4181c07Operated by a computer and powerful software allows user to program any type of exposure with all variables and repeat those exposures with the click of a mouse. Profiles can be created, stored and recorded 24 hrs a day/7 days a week.

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oxycyclergt4181c09Hypoxic stress can model components of many severe diseases such as heart attacks, strokes, asthma, or epilepsy. Frequency, duration and degree of drops are all adjustable.

oxycyclergt4181c11Model step reduction in oxygen, similar to altitude acclimation, to gradually condition cells for hypoxic upregulation of gene expression. The rate of change between any two levels is adjustable and repeatable. The duration at any given level is adjustable and repeatable.

oxycyclergt4181c10Cells destined for implantation will experience hypoxia and may be better prepared if they are conditioned to it before implantation. Ischemia may also be protected by conditioning. The OxyCycler GT4181C can easily run any preconditioning profile.

oxycyclergt4181c12Sudden increases in oxygen can cause cell damage. The OxyCycler GT4181C allows modeling toxicity of oxygen in any cell culture, similar to toxicity from recreational oxygen inhalation by athletes, therapeutic oxygen administration in critical care units, and other sudden exposures to high oxygen. The rate of oxygen increase can be adjusted to change faster to overwhelm antioxidants, or change slower to condition for antioxidants.