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C-SHUTTLE

Glove Box

  • Full Time O2/CO2 Optimization During Handling
  • Full Time Protection
  • Better Cell Growth
  • Better Cell Health
  • Maximum User Comfort
  • Ample Workspace
  • Accommodates Subchambers
  • Hypoxia in vitro models
  • Physiologic oxygen for cell culture

CYTOCENTRIC HOOD

The C-Shuttle laboratory glove box is part of a practical system for handling your cells under the same conditions at which they incubate. It provides ample workspace where you can handle and manipulate your cells without disruption of O2 and/or CO2.

FULL TIME OPTIMIZATION DURING CELL HANDLING

Interruption in optimal O2 and/or CO2 conditions can have major effects on cell cultures. Normally when cells are manipulated outside their incubator in clean hoods, controlled gases such as CO2 and O2 are disrupted. Effects can be devastating on cultures. Consider oxygen regulated genes. Hypoxia upregulated genes are immediately shut off by even brief exposures to room air. Imagine what can happen to receptors, ion channels, cytokines, extracellular matrix, etc. when the pH changes out of the physiologic range!

DOCKS SUBCHAMBERS FROM REMOTE INCUBATORS

Using a transport vessel such as the BioSpherix C-Chamber between the C-Shuttle laboratory glove box and your incubator is the most economical way to achieve uninterrupted conditions. The incubator protects cells from exposure. The transport chamber maintains conditions for the brief period between controlled environments. The C-Shuttle hypoxia work station is controlled, and the buffer chamber allows your cells to enter without disrupting the set environment within.

 

Parts

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COMPLETE SYSTEM

The C-Shuttle, a C-Chamber (sold separately), a gas controller (system comes with ProOx P360, other options available) and gas (customer supplied). A nitrogen gas supply is necessary for hypoxia. If CO2 control is necessary, a CO2 controller and tank of CO2 is also required. site iglove10

CONTROLLERS

Controllers such as the ProOx P360 (pictured above) and similar O2 / CO2 controllers fit the C-Shuttle. They are flexible and very capable. site iglove11 Three standard C-Chambers sizes are compatible with the C-Shuttle. One of the three should fit most applications.

Options

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Typical configuration for one user access (Top view)

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Optional two user access configuration (Top view)

Dimensions

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Front View

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Buffer End View

Installation Schematic

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Installation

1. Set C-Shuttle on Secure level surface. 2. Place controller(s) on or near buffer chamber. 3. Hook up gas supply. 4. Connect the controller(s), power supply and sensor

How It Works

cshuttle04The unique C-Shuttle Glove Box is a component of the SubChamber System. It provides a workspace for cell culture processing under the same O2/CO2 conditions that the cells live, while in the incubator. Another component, the C-Chambers, provide a gas tight transport of cultures from the incubator to the workspace and back to the incubator. The pass through chamber, or antechamber, of the glove box is designed to fit C-Chambers. You can leave C-Chambers in the antechamber, and remove cells into uninterrupted O2/CO2 atmosphere, manipulate them without disturbance, and finally return them into the incubator with the same O2/CO2. cshuttle05

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Cells are moved into the buffer chamber protected inside a subchamber with opening facing the workspace.

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Technician removes cells from the subchamber docked in the buffer chamber.

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Cells are handled and manipulated under aseptic and optimal O2/2 conditions and returned to subchamber after handling.

cshuttle09Cells removed from normal incubator and handled in normal hood are exposed to suboptimal conditions. Cells cultured in a subchamber and handled in a C-Shuttle are never exposed to suboptimal conditions. cshuttle10The C-Shuttle allows plenty of head-space for long pipettes and other instruments. Technicians work comfortably.
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C-Shuttles protect the researchers

Cells are often purposely grown and processed to generate or test viruses and vectors. More worrisome is when cells are unknowingly infected with viruses or other transmissible agents. C-Chambers will limit exposure to harmful viruses, vectors and prions where conventional open labs allow the technicians to breathe potentially dangerous non-cellular biological entities.

Uninterruptibility

Cells removed from normal incubator and handled in normal hood are exposed to suboptimal conditions. Cells cultured in a subchamber and handled in a C-Shuttle are never exposed to suboptimal conditions.

PROTECTION AGAINST CONTAMINATION

In open labs with open hoods, cells and researchers share the critical workspace most of the time. The majority of contamination in labs is caused by humans and their bioburden. A closed environment provided by the C-Shuttle and C-Chambers ensures that the main source of contamination is removed.

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