Stem Cell
Learn how stem cell cultures benefit from physiologic oxygen with the information below.
Oxygen is a fundamental part of the stem cell niche. Physiologic O2 levels are far below those provided in most stem cell experiments (room air – 21% oxygen). In the body, stem cells experience a range of oxygen levels: mesenchymal stem cells (MSCs) range 2-8% O2, hematopoietic stem cells (HSCs) at 1-6% oxygen, and neural stem cells (NSCs) from 1-8% O2. Human development occurs under low oxygen conditions and scientists optimize O2 conditions (2-5% O2) for experiments with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Multiple benefits are reported for physiologic oxygen in stem cell cultures:
- increase proliferation
- maintain pluripotency
- promote proper phenotype
- streamline cell reprogramming
- preserve stemness
- increase yield
- diminish spontaneous differentiation
- decrease doubling time
- boost self-renewal
- control lineage commitment
- modulate quiescence
- enhance repopulating ability
Most stem cell experiments are performed under conditions never found in the body.
Researchers are using new tools for culture, handling, and analysis of stem cells under biologically relevant oxygen levels (or physioxia). Upgrading existing CO2 incubators with insertable O2-controlled chambers is an economical approach for optimizing oxygen in stem cell experiments. Even brief interruptions of physiologic O2 during culture maintenance in open hoods/BSCs alter stem cell function. To prevent this, physioxia (or hypoxia) workstations maintain constant control of O2, CO2, and Temp with interconnected incubators and hoods for all steps of cell incubation, handling, and analysis.
Cell Equipment
ProOx C21 & C-Chamber
Cell Research – physioxia / hypoxia chamber with static control of O2 and CO2 – ProOx C21 & C-Chamber
OxyCycler C42 & C-Chamber
Cell Research – physioxia / hypoxia chamber with dynamic control of O2 and CO2 – OxyCycler C42 & C-Chambers
Xvivo System
Cell Research – physioxia / hypoxia workstation, combined oxygen incubator and hood system, in vitro oxygen glove box, with independently programmable control of O2, CO2, and Temp in one (or more) modular incubator chamber and/or hood chambers – Xvivo System
Discover More
Blog Posts
Cytocentric Blog – Interview with Cytocentric Visionary: Roger Rönn – The Cell Environment for HSC from iPS: Why Every Hurdle Must be Overcome to Reach our Destination
Cytocentric Blog – Why Optimize MSC Cell Culture Conditions? Feed a Fibroblast, Starve a Stem Cell
Cytocentric Blog – Interview with Cytocentric Visionary: Dr. Heather O’Leary – Part One: Extraphysiologic Oxygen ShockPart One: Extraphysiologic Oxygen Shock
Posters
BioSpherix 2017 ISSCR – Full-Time Physioxic Control Maximizes Human MSC Expansion at the Individual Cell and Population-wide Levels
BioSpherix 2017 Perinatal Stem Cell Society – Cord Blood Hematopoietic Stem Cell Function is Affected by In Vitro Oxygen
Publications
Browse 700+ Stem Cell Research publications citing oxygen equipment from BioSpherix Ltd
Lab References
Stem Cell Research at University of Toulouse
Stem Cell Research at Centre for Innovative Medicine
Stem Cell Research at University of Bordeaux
Stem Cell Research at SUNY Upstate Medical University
Videos
Avoid Irrelevant Non-Reproducible Data, The Neglected Variable: Physiologic
Whitepapers
Full-time Control of Conditions During Cell Handling Enhances the Growth of Human Mesenchymal Stromal Cell Cultures Alicia D. Henn1, Claudia Schwartz2, Shannon S. Darou1, Randy Yerden1 1 BioSpherix Ltd., Parish, NY, USA 2 Lonza Cologne GmbH, Koeln, Germany