Oxygen is an important physiological parameter in healthy neural tissues. Levels range 1-6% O2 for neurons and glial cells. They never experience the 21% O2 (room air oxygen) used in most cell-based neuroscience studies. Since oxygen modulates neural cell function, use of physiologic oxygen (or physioxia) during in vitro experiments is helpful for adding physiological relevance.
- mitochondrial function of astrocytes
- differentiation of oligodendroglial cells
- dopaminergic neurogenesis
- neuronal cell differentiation
- expansion of neural progenitors
- neural progenitor fate
- dopaminergic differentiation
- oligodendrocyte lineage cell yields
- neuronal metabolism
- proliferation of mesencephalic precursor cells
- transcriptional regulation in astrocytes
- neurite outgrowth
- expansion of neural precursor cells
Pathological oxygen levels (hypoxia or hyperoxia) are used for in vitro / in vivo modeling of neural diseases and disorders: Alzheimer’s Disease, Attention Deficit Hyperactivity Disorder (ADHD), brain ischemia, stroke, Amyotrophic Lateral Sclerosis (ALS), ischemia-reperfusion, oxygen glucose deprivation (OGD), neurodegeneration, gliomas (including glioblastoma), neuroplasticity, hypoxic-ischemic brain injury, sleep apnea, and neurodevelopmental disorders. Elevated CO2 (hypercapnia) is used for modeling anxiety or panic disorders.
The physiologic oxygen chamber, ProOx C21 & C-Chamber is a subchamber system allowing researchers to add control of specific O2 levels within existing cell incubators without increasing the laboratory’s equipment footprint. Adding two or more ProOx C21 & C-Chamber oxygen chambers helps provide simultaneous control at multiple oxygen levels over the full range of physiologic values.
OxyCycler C42 & C-Chamber
The Xvivo System from BioSpherix Ltd is a physiologic oxygen workstation combining one or more O2 incubators with attachable closed hoods. This provides non-varying physioxic conditions during the entire cell experiment. The Xvivo System may be outfitted with multiple O2 incubators so investigators can perform experiments at different O2 tensions at the same time. The Xvivo System’s modular physioxia hoods are connected to the incubator chamber(s) to permit cell handling and cell analysis under the same conditions found in the incubator.
OxyCycler A84XOV & A-Chamber
Use the OxyCycler A84XOV & A-Chamber for creating in vivo exposures with intermittent hypoxia, graded hypoxia, hypoxia/reoxygenation, and hypoxia/hyperoxia swings. Control dynamic O2 levels in 1-4 chambers at the same time. Optional Monitor Pod records environmental conditions inside chamber: parts per million carbon dioxide (CO2 ppm), relative humidity (RH), temperature, and remote oxygen.
Quick & Quiet Profiler
To better mimic pathophysiology, add the Quick & Quiet Profiler accessory kit to OxyCycler A84XOV & A-Chamber or OxyCycler A41OV & A-Chamber to accelerate the exhange between hypoxia and ambient room air (normoxia) while maintaining sound levels (dB) within acceptable ranges.
Jaber SM, Bordt, EA, et al. Sex differences in the mitochondrial bioenergetics of astrocytes but not microglia at a physiologically relevant brain oxygen tension. Neurochem Int, 2017. 2018 Jul;117:82-90.
Neuroscience Research at Western University
Neuroscience Research at McGill University