Cancer / Tumor Biology
Hypoxia is a well known aspect of cancer biology. The Warburg Effect describes how tumor cells use metabolic pathways adapted to low oxygen conditions (0-4% O2) in poorly vascularized, highly competitive tumor environments. Pathophysiologic hypoxia is a contributing factor in carcinogenesis, cell cycle, metabolism, angiogenesis, metastatic progression, induction of stem-like traits, immune evasion, and therapeutic resistance.
To understand hypoxia’s role in cancer cell phenotype, researchers use hypoxia in cell-based experiments to simulate the cancer microenvironment. Physiologically relevant oxygen environments improve the predictive capacity of cell-based assays. Cancer scientists insert hypoxia chambers into existing CO2 incubators to economically perform low oxygen (hypoxic) experiments without purchasing new incubators.
Brief interruptions of hypoxia occur when handling cell cultures in open hoods and these disruptions alter cell function. Instead, using hypoxic workstations (glove boxes) to incubate and handle tumor cells prevents the random variation of oxygen conditions that normally occur during cell manipulation and cell analysis.
Most cell-based cancer studies are performed with oxygen conditions never found in tumors.
BioSpherix Ltd provides hypoxia chambers and hypoxia workstations designed to help cancer scientists increase both the reproducibility of experiments and the relevance of their data. The hypoxic subchamber system ProOx C21 & C-Chamber upgrades existing CO2 incubators with control of specific O2 levels. Add two or more ProOx C21 & C-Chamber hypoxia chambers to provide simultaneous control at multiple oxygen levels over the full range of pathophysiologic oxygen.
The Xvivo System from BioSpherix Ltd is a hypoxia workstation that combines one or more O2 incubators with attachable closed hoods. This provides non-varying hypoxic conditions during the entire cell experiment. The Xvivo System may be outfitted with multiple O2 incubators so investigators can perform experiments at different O2 tensions at the same time. The Xvivo System’s modular hypoxia hoods are connected to the incubator chamber(s) to permit cell handling and cell analysis under the same conditions found in the incubator.
Dumas PY, et al. Dual Inhibition of FLT3 and AXL by Gilteritinib Overcomes Hematopoietic Niche-Driven Resistance Mechanisms in FLT3-ITD Acute Myeloid Leukemia. Clin Cancer Res. 2021;10.1158/1078-0432. doi:10.1158/1078-0432.CCR-20-3114
Cancer Research at University of Bordeaux
Cancer Research at Aurelia BioScience Ltd.