Toxicology – In Vitro

Oxygen is an important regulator of cell function. Physiologic O₂ (or physioxia) is much lower than oxygen levels provided in most cell-based toxicology assays (room air – 21% oxygen). In the body, cells experience a range of tissue O₂ levels but they never endure room air: liver (3-9%), brain (1-6%), heart (2-5%), lung (10-13%), skin (1-4%), kidney (1-6%), bone marrow (0.1-7%), vascular (2-5%),and retinal (2-5%).

Oxygen regulates the toxicity response of:

Toxicologists are using new tools to culture, handle, and analyze in vitro toxicity assays under biologically relevant conditions. To enhance in vitro-in vivo correlation, the incubator subchamber is an economical option that adds O₂ control to CO₂ incubators. Handling cells outside the incubator in open BSCs/hoods disrupts the in vivo-like conditions provided in the physioxia chamber. These interruptions diminish assay reproducibility and they are prevented by performing the entire in vitro toxicology workflow under continuous, non-interrupted physiologic conditions. Physioxia workstations combine one or more individually controlled incubators with closed hoods permitting continuous control of assay conditions (O₂, CO₂, and Temp) during all steps of cell incubation, handling, and analysis. Now that you have learned more about oxygen regulation with in vitro toxicology, discover some BioSpherix equipment options below.

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Cell Equipment

ProOx C21 & C-Chamber

The physioxic incubator subchamber, ProOx C21 & C-Chamber allows researchers to add O₂ control to existing CO₂ incubators without increasing the laboratory’s equipment footprint. Use two or more ProOx C21 & C-Chamber oxygen chambers to provide simultaneous control at multiple physiologic O2 levels.

Xvivo System

The Xvivo System from BioSpherix Ltd is a physioxic workstation that combines one or more O₂ incubators with attachable closed hoods. This provides non-varying physiological oxygen conditions during the entire cell experiment. The Xvivo System may be outfitted with multiple O₂ incubators empowering investigators with the ability to perform experiments at multiple, different O₂ tensions at the same time. The Xvivo System’s modular physioxia hoods are connected to the incubator chamber(s) to permit cell handling under the same conditions found in the incubator.

Plate Readers, Real-time Imagers, & Cell Analyzers

Enclose platforms like:

Phase Holographic Imaging Holomonitor

The Xvivo System’s modular physiologic oxygen hoods enclose 3rd-party instruments in order that well plates may be read, imaged, and analyzed under the same conditions found in the incubator.