Toxicology - In Vitro

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Oxygen is an important regulator of cell function. Physiologic O2 (or physioxia) is much lower than oxygen levels provided in most cell-based toxicology assays (room air - 21% oxygen). In the body, cells experience a range of tissue O2 levels but they never endure room air: liver (3-9%), brain (1-6%), heart (2-5%), lung (10-13%), skin (1-4%), kidney (1-6%), bone marrow (0.1-7%), vascular (2-5%),and retinal (2-5%).

Oxygen regulates the toxicity response of:

Toxicologists are using new tools to culture, handle, and analyze in vitro toxicity assays under biologically relevant conditions. To enhance in vitro-in vivo correlation, the incubator subchamber is an economical option that adds O2 control to CO2 incubators. Handling cells outside the incubator in open BSCs/hoods disrupts the in vivo-like conditions provided in the physioxia chamber. These interruptions diminish assay reproducibility and they are prevented by performing the entire in vitro toxicology workflow under continuous, non-interrupted physiologic conditions. Physioxia workstations combine one or more individually controlled incubators with closed hoods permitting continuous control of assay conditions (O2, CO2, and Temp) during all steps of cell incubation, handling, and analysis.