Xvivo System Model X3
The Hypoxia Incubator and Glovebox Configuration of the Xvivo System model X3 is a simple but sophisticated hypoxia cell culture system. It is the most economical X3 configuration available with identical incubation and handling oxygen tensions. Modular, independent controls and components deliver all essential functions, yet offer unlimited upgrade options.
Unlike bacteria glove boxes cobbed up for cells, the X3 system was designed for cells. It is economically competitive with all bacteria hypoxia glove boxes, but offers much more capability including active particle clearing to ISO 5. Features comfortable, soft glove sleeves as opposed to hard, uncomfortable holes. Ample head room allows for easy pipetting and use of other equipment and accessories.
Docking for remote culture chambers and transport chambers allows you to transport cells from the closed hood to other areas without risk of contamination. Expand culture capacity infinitely. Use culture chambers to receive cells from every incubator in your lab.
Cytocentric-by-Design ushers in new era for cell scientists at the leading edge!
New and Better Equipment
Conventional incubators and hoods haven't changed in decades. They are limited in ability. They were designed for people, not cells. Now a radical new alternative type of equipment is available for manipulating and growing cells. It is unlike any other equipment. The Xvivo System is the first system designed to go beyond the ordinary needs of people and specifically address the advanced needs of cells. Better for your cells. Better for you.
From the Perspective of Cells
When you look through the eyes of your cells, handicaps inherent in conventional People-Centric equipment are obvious. Protection from contamination is poor. Critical parameters are not accounted for, or poorly controlled at best. Eliminating these handicaps and better meeting the needs of cells requires something different. For people, the Xvivo System may be a radical new concept. For cells, it's not. For cells, it is about time.
Cell Incubation and Processing Re-Invented
In contrast to conventional cell culture and processing in open incubators and open hoods, the Xvivo System consists of modular sets of closed incubators and closed hoods, all integrated together as co-chambers and sub-chambers. Modularity allows an infinite variety of different configurations. All are completely closed, with aseptic conditions throughout, and advanced controls wherever needed. Microscopes, centrifuges, sorters, and all other common cell tools can be integrated as well. For the first time, cell needs can be met by one efficient system.
High Performance Incubators
Incubation chambers are integrated into the hoods where you need them, and open only into the hood. This eliminates 2 major handicaps in conventional open incubators and open hoods. First, incubators can't get contaminated by people or by the room. They are isolated from both. They open only into the aseptic hood. Second, cells never see any disruptions in critical cell parameters, because all cell parameters controlled in incubators are also identically controlled in hoods. Any number of independent incubation chambers can be fitted into a system. Each can be sized for and dedicated to each different culture to accommodate any number of different culture protocols simultaneously. Basic cell parameters like O2 are fully accounted for, and exotic new cell parameters not available elsewhere provide better simulation of physiologic conditions. Every parameter can have dynamic control to keep up with the changing dynamics of any cell population.
Expansion Protocol - Primary cultures and other low cell population density do best under low oxygen gas phase when they start, but when they grow, they are bound to need more. You can design any ramp up in oxygen, and consistently repeat it. With a click of the mouse.
Acute/Intermittent Hypoxia - Hypoxic stress can model components of many severe diseases such as heart attacks, strokes, asthma, or epilepsy. Frequency, duration and degree of drops are all adjustable.
Hypoxic Preconditioning - Cells destined for implantation will experience hypoxia and may be better prepared if they are conditioned to it before implantation. Ischemia may also be protected by conditioning. The OxcyCycler C42 can easily run any preconditioning profile.
Hypothetical Protocol - To illustrate the unprecedented power of dynamic multi-variable optimization, imagine you have a transgenic cell population to be implanted. You use the last few hours before implantation to prepare it. You know the cells are going to better survive the time between the dish and the body if their metabolism slows down, so you program the temperature to gradually cool the cells just before they leave the incubator. You know your cells are going to be hypoxic for their first few days in the body, so you program the O2 for a series of acute hypoxic episodes in the last hours before implantation to precondition them, then at the last moment make the O2 rise to saturate their media just before they leave the incubator. You also know they will handle the stress a lot better if you can upregulate their stress response, so you program a short burst of CO just before they leave the incubator. As usual, you program the CO2 concentration to stay constant while the other variables are manipulated up and down.
High Performance Integrated Hoods
Hoods are closed instead of open. Isolation of cells away from people and the room cuts risk of contamination during handling dramatically. Multiple levels of aseptic conditioning provides a more cleaner cell environment than any conventional BSL Class 100 hood. All manual and automated processing is protected from contamination. All incubators are protected because they open only into these isolated protected aseptic hoods. All cells are protected. All the time. Hoods can have some or all the same cell parameters as the incubators. Now it is easy to handle and process cells with no disruption in optimum conditions.
Xvivo System modularity means you can invest with confidence. Start small and expand as necessary. Reconfigure if your needs change. No dead ends. Xvivo System can grow with you. Modularity allows you to invest in just what you need now, and then add to it later when your needs change or expand.
- Step One: Starter system with one closed hood for culture processing and one incubation chamber on end.
- Step Two: Same starter system: moved incubator to back wall, added a microscope chamber (open and contiguous with culture processing chamber), and another processing chamber for second technician and 3rd party equipment like cell separator, colony picker, plate reader, etc.
- Step Three: Added 12 additional incubation chambers (8 in one hood, 4 in the other) for a new total of 13, each independently controlled, and added a couple of small laminar flow clean hoods one at each end. Modularity means you will never be limited.