Physiologic Oxygen / Physioxia
Deepen your understanding of how physioxia affects cell function and scientific reproducibility below. Oxygen is an important modulator of cell function yet most in vitro research occurs under Supraphysiologic levels (ambient air – 21% O2) that misrepresent the in vivo tissue setting and lead to irrelevant results. Normal tissue oxygen levels are far below those found in air and the often misused term ‘hypoxia’ should be reserved only for reporting pathologically low oxygen. The Term ‘physioxia’ is used more frequently to describe biologically relevant tissue O2 levels. Oxygen affects cell function in many ways:
- metabolic activity
- proliferation rate
- differentiation / dedifferentiation
- gene expression
- cell cycle / DNA replication integrity
- stress response
- homeostatic capacity
- redox state
- signaling cascades
- simulate physiological cellular O2 levels
Default Room Oxygen adds ROS Artifact to Cell Culture.
New tools are available for incubation, handling, and analysis of cell cultures under full-time physiologically relevant oxygen levels (physioxia). Upgrade CO2 incubators with O2-controlled subchambers. Since brief interruptions of physioxia affect scientific reproducibility, physiologic oxygen workstations (or glove boxes) increase data consistency through constant control of O2, CO2, and Temp during every step of the cell experiment.
OxyCycler C42 & C-Chamber
Cell Research – physiologic oxygen incubator and hood system, physioxia workstation, physioxia glove box with independently programmable control of O2, CO2, and Temperature in one (or more) modular incubator chamber(s) and/or cell handling chamber(s) – Xvivo System
CytoCentric Blog – What Oxygen Level Should I Use for My Cells In Vitro?
CytoCentric Blog – What Oxygen Level Does My Cell Culture Actually Experience?
CytoCentric Blog – Oxygen Terminology
*new* Interview with Cytocentric Visionary: Dr. Sandeep Raha from McMaster University – Physiologic O2 for In Vitro Placental Research Reveals New Understanding
*new* Wong, Michael K et al. , Establishment of an in vitro placental barrier model cultured under physiologically relevant oxygen levels. Molecular human reproduction vol. 26,5 (2020): 353-365. doi:10.1093/molehr/gaaa018.
Physiologic Oxygen at University of Virginia
Physiologic Oxygen at Sanford Burnham Prebys Medical Discovery Institute
Physiologic Oxygen at SUNY Upstate Medical University