Physiologic Oxygen / Physioxia

Deepen your understanding of how physioxia affects cell function and scientific reproducibility below. Oxygen is an important modulator of cell function yet most in vitro research occurs under super-physiological levels (ambient air - 21% O2) that misrepresent the in vivo tissue setting and lead to irrelevant results. Normal tissue oxygen levels are far below those found in air and the often misused term ‘hypoxia’ should be reserved only for reporting pathologically low oxygen. The Term 'physioxia' is used more frequently to describe biologically relevant tissue O2 levels. Oxygen affects cell function in many ways:

  • metabolic activity
  • proliferation rate
  • differentiation / dedifferentiation
  • gene expression
  • cell cycle / DNA replication integrity
  • stress response
  • homeostatic capacity
  • redox state
  • signaling cascades
  • implantation
  • simulate physiological cellular O2 levels

New tools are available for incubation, handling, and analysis of cell cultures under full-time physiologically relevant oxygen levels (physioxia). Upgrade CO2 incubators with O2-controlled subchambers. Since brief interruptions of physioxia affect scientific reproducibility, physiologic oxygen workstations (or glove boxes) increase data consistency through constant control of O2, CO2, and Temp during every step of the cell experiment.