Physiologic Oxygen / Physioxia
Oxygen is an important modulator of cell function yet most in vitro research occurs under super-physiological levels (ambient air - 21% O2) that misrepresent the in vivo tissue setting and lead to irrelevant results. Normal tissue oxygen levels are far below those found in air and the often misused term ‘hypoxia’ should be reserved only for reporting pathologically low oxygen. The Term 'physioxia' is used more frequently to describe biologically relevant tissue O2 levels. Oxygen affects cell function in many ways:
- metabolic activity
- proliferation rate
- differentiation / dedifferentiation
- gene expression
- cell cycle / DNA replication integrity
- stress response
- homeostatic capacity
- redox state
- signaling cascades
- implantation
- simulate physiological cellular O2 levels
New tools are available for incubation, handling, and analysis of cell cultures under full-time physiologically relevant oxygen levels (physioxia). Upgrade CO2 incubators with O2-controlled subchambers. Since brief interruptions of physioxia affect scientific reproducibility, physiologic oxygen workstations (or glove boxes) increase data consistency through constant control of O2, CO2, and Temp during every step of the cell experiment.
Equipment Options
ProOx C21 & C-Chamber
Cell Research - physiologic oxygen, chamber, physioxia chamber with static control of O2 and CO2 - C21 & C-Chamber
OxyCycler C42 & C-Chamber
Cell Research - physiologic oxygen chamber(s), physioxia chamber(s) with dynamic control of O2 and CO2 - C42 & C-Chambers
Xvivo System
Cell Research - physiologic oxygen incubator and hood system, physioxia workstation, physioxia glove box with independently programmable control of O2, CO2, and Temperature in one (or more) modular incubator chamber(s) and/or cell handling chamber(s) - Xvivo System
