It’s All About the Cargo: O2 Levels Change Exosome Signaling
Jiang et al. summarize the cargo changes and biological effects of hypoxia on exosomes.
Regenerating Muscle Requires Physioxia
Elashry et al. find that exposure to low oxygen promotes the multipotency of skeletal muscle-derived stem cells, benefitting tissue regeneration.
Oxygen as a Signaling Molecule
Early erythroid cell fate is influenced by oxygen concentration, as shown by Labat et al. of Universite de Bordeaux.
Read more: https://www.mdpi.com/2218-273X/12/4/540
Corneal Endothelial Cells Behave Differently at Physioxia
Patel et al. evaluate the metabolism of HCEnCs cultured at physiological O2 and room air.
Read more (open access): https://tvst.arvojournals.org/article.aspx?articleid=2778592
Using the GBM Microenvironment to Regulate Plasticity
Uribe et al.’s review of glioblastomal adaptation details plasticity regulation in hypoxic niches.
Read more (open access): https://www.mdpi.com/2079-7737/11/2/313
Advancing Cell-Based Meniscal Therapies with the Use of Physioxia
Pattappa et al. present further evidence of the enhanced clonogenicity and differentiation potential of meniscus cells under physiologic O2.
Read more (open access): https://www.frontiersin.org/articles/10.3389/fbioe.2021.789621/full
Hypoxia Unmasks New Therapeutic Targets
Clees et al. detail how physiologic hypoxia alters the regulation of nine distinct proteins in multiple myeloma cells.
Read more (open access): https://www.mdpi.com/2073-4409/11/2/292
Landmark Paper Demonstrates Environmental Instability in Cell Culture
Another breakthrough paper from Klein et al. has demonstrated a pervasive issue affecting in vitro experimental findings: large fluctuations of environmental conditions during standard batch culture.
Read more (open access): https://www.nature.com/articles/s42003-022-03065-w
Modeling Preeclampsia with Oxygen In Vitro
Strutz et al. model fetal hypoxia and find memory effects on angiomiR expression in fpEC.
Read more (open access): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705946/
Culturing Under Hypoxia Reveals Prostate Cancer Growth Regulator
Rios-Colon, et al. of Wake Forest Baptist Medical Center demonstrate how Carnitine Palmitoyltransferase 1 regulates PCa survival and aggressiveness under hypoxic conditions.
Read more (open access): https://www.mdpi.com/2072-6694/13/24/6302
Hypoxic Conditions Stabilize HIF-1a in Brain Endothelium
University of Copenhagen authors Ozgur et al. detail how hypoxia increases expression of some blood-brain barrier transporters in brain capillary endothelial monolayers
Read more (open access): https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-021-00297-6
Culturing Under Physioxia Improves Human Embryo Quality and Development
Herbemont et al. find more evidence that encourages use of a physiologic O2 environment for human embryos at cleavage stage.
Read more (open access): https://www.nature.com/articles/s41598-021-01488-9
Guidance on Good Cell Culture Practices 2.0 is Now Available
The updated guidance document addresses quality management in a new age of research, providing insight into reproducible methods for in vitro cell culture.
Read more (open access): https://www.altex.org/index.php/altex/article/view/2376
Using Oxygen to Control Differentiation
A comprehensive review by Hu et al. highlights hypoxia as a tool for modulating chondrocyte redifferentiation.
Read more (open access): https://www.frontiersin.org/articles/10.3389/fbioe.2021.764193/full
Hypoxic Cell Culture Used to Identify Therapeutic Targets
By characterizing PASMCs cultured under hypoxia, He et al. examine the critical role vascular cell metabolism plays in hypoxic pulmonary hypertension.
Read more (open access): https://www.frontiersin.org/articles/10.3389/fphar.2021.753727/full
New Data Spotlights Need for Greater Rigor and Transparency
The long-awaited conclusion of the Reproducibility Project: Cancer Biology. Only 46% of reported effects could be replicated across 50 high-impact studies.
Read more: https://elifesciences.org/articles/71601
Glioblastoma Activity is Altered by Hypoxia
Pathophysiologic oxygen induces morphological changes, increased expression of anti-apoptotic and proliferation markers, and miRNA expression dysregulation in GBM cells.
Nature BME Publishes Urgent Call for Reproducibility
A landmark article by Klein et al. calls for widespread change in environmental control in mammalian cell culture.
Metabolic Profile of Circulating ILC2s Depends on OXPHOS/O2
Surace et al. demonstrate complete control of O2 and physiologic parameters as they expand current knowledge of ILC2 metabolism in this notable publication in Nature Immunology.
Closed Manufacturing Platforms are Crucial for Cell Therapy Revolution
Abraham et al. assert that the next step towards commercial-scale autologous cell therapy manufacturing is closed, automated, and scalable platforms.
A Cytocentric Giant: Hal Broxmeyer
In December, we lost a mighty scientist who founded modern cord blood medicine, and showed the whole world how constant physiologic oxygen is critical to cells during isolation.
Oxygen Intrinsically Linked to Cell Senescence
Wiley and Campisi delve into the myriad causes of cellular senescence, including the influence of oxygen on senescent cell phenotype and frequency.
Congratulations! Join the movement:
MSC Therapy is Improved by Physiologic O2
A comprehensive review by Egger et al. concludes that physiologic O2 levels maintain or increase the therapeutic properties of MSCs, and calls for reduced oxygen conditions during isolation and expansion of cells for cell products.
Read more (open access): https://onlinelibrary.wiley.com/doi/full/10.1002/elsc.202100097
Anticancer Drug More Potent at Physioxia
Otto-Ślusarczyk et al. of the Medical University of Warsaw find greater effectiveness of Teriflunomide at physiological oxygen levels, suggesting in vitro studies at room-air O2 could be underestimating drug potency.
Read more (open access): https://www.sciencedirect.com/science/article/pii/S2405580821002351?via%3Dihub
Physiologic Oxygen Decides Placental Development, Pregnancy Outcomes
A review of the critical role physioxia plays in development and maternal immunity during pregnancy, by Hui Zhao et al. of Stanford University School of Medicine.
Read more (open access): https://www.mdpi.com/1422-0067/22/18/9675
Hypoxia Induces Quiescence In Vitro in Treatment-Resistant Leukemic Cells
O’Reilly et al. of the Apoptosis Research Centre create models of quiescent leukemic stem cells by mimicking the bone marrow microenvironment, leading to a potential method of high-throughput drug screening.
Read more (open access): https://www.frontiersin.org/articles/10.3389/fcell.2021.662868/full
O2 Tension Alters Chemical Sensitivity in ESC Culture
RIVM’s Mennen et al. demonstrate the influence of oxygen level on the chemical sensitivity of the ESTc, a model for developmental toxicity predictions.
Physioxia Enhances the Pro-Regenerative Activity of MSCs
Zhidkova et al. of the Russian Academy of Sciences detail the crosstalk betwen ECs and MSCs co-cultured under physiological hypoxia, and how culturing under physiologically relevant conditions increases migration and anti-inflammatory modulation.
Click here to read more (open access): https://www.mdpi.com/2673-8937/1/2/9/htm
Oxygen Levels Influence Cytokine Responses
University College Dublin’s Malkov et al. review the direct impact of cytokines on HIF signaling, and the multi-level crosstalk that shapes hypoxic inflammation.
Click here to read more (open access): https://www.mdpi.com/2073-4409/10/9/2340/htm
Nitric Oxide Mimics the Hypoxia Response to Enhance Stemness
Caballano-Infantes et al. dissect the stabilization of the HIF pathway in NO-treated iPSCs and ESCs
Click here to read more (open access): https://www.mdpi.com/2076-3921/10/9/1408/htm
Cytocentric Principles in Clinical Cancer Research: Mimicking the Hematopoietic Niche
Congratulations to Pierre-Yves Dumas et al. at the University of Bordeaux on their publication in Clinical Cancer Research, in which they used physiologic oxygen to model the tumor microenvironment in vivo.
Click here to read more: https://clincancerres.aacrjournals.org/content/early/2021/09/21/1078-0432.CCR-20-3114.long
Hypoxia As a Crucial Regulator of Muscle Vascularization
York University authors Pierre Lemieux and Olivier Birot review the relationship between sources of cellular hypoxic stress in the body, and the vascularization of muscle tissue in the presence of that stress.
Click here to read more (open access): https://www.frontiersin.org/articles/10.3389/fphys.2021.735557/full
Mechanical Loading with Physioxia Enhances Cartilage in Vitro
Lead author Alexander Szojka and colleagues at the University of Alberta investigated the effects of “mechano-hypoxia conditioning” on gene expression in engineered human meniscus tissues.
Click here to read more (open access): https://www.frontiersin.org/articles/10.3389/fbioe.2021.739438/full
O2 Regulates Pluripotent Stem Cell Niche, Metabolism, Differentiation, and Reprogramming Efficiency
Jagiellonian University authors Kinga Nit, Malgorzata Tyszka-Czochara, and Sylwia Bobis-Wozowicz offer a comprehensive review of the critical role oxygen plays in the function and metabolism of PSCs and CSCs.
Click here to read more (open access): https://www.mdpi.com/2075-4426/11/9/905
Oxygen-Controlled In Vitro Study Confirms Mechanism of Diabetic Vascular Disease
Congratulations to lead author Min Zhao and colleagues at the Southern Medical University on their publication of novel evidence linking the HIF-1a/JMJD1A signaling pathway to oxidative stress following hyperglycemia.
Click here to read more (open access): https://cmbl.biomedcentral.com/articles/10.1186/s11658-021-00283-8
Hypoxia Research Reveals Novel Diagnostic Tool for Cancer
Lead author Alicja Gluszko and colleagues discovered distinct proteomic profiles in hypoxic small extracellular vesicles, which could be used to monitor tissue hypoxia in head and neck squamous cell carcinoma.
Click here to read more (open access): https://www.mdpi.com/2072-6694/13/16/4176/htm
Same Donor Cells, Different O2: Controlling Oxygen Allows Researchers to Promote Chondrogenesis in MSC Culture
An exciting publication in Cells from Dr. Graziana Monaco et al, in which they used oxygen tension to tailor the differentiation of mesenchymal stromal cells in vitro.
Click here to read more (open access): https://www.mdpi.com/2073-4409/10/8/2165
Mitigating Neonatal Injury: Hypoxia Research Leads to New Potential Therapies
Congratulations to lead author Darren Bodkin and colleagues from SUNY Downstate Medical Center and SUNY Eye Institute on their publication in Pediatric Research, in which they demonstrated that antioxidants and fish oil may mitigate Intermittent-Hypoxia-induced injury to the gut.
Read the full paper here (open access): https://www.nature.com/articles/s41390-021-01707-z
Oxygen and HIF May Hold the Key to Improving Outcomes of Hematopoietic Disorders
Breakthrough Science: 2019 Nobel Laureate Gregg Semenza’s review of the connections between the oxygen sensing pathway and disorders of the blood.
Simulating the Bone Marrow Microenvironment In Vitro Provides Insight on Anti-Leukemic Therapies
Congratulations to first author Pierre-Yves Dumas and team at University of Bordeaux on their findings first published August 13 in Clinical Cancer Research establishing that single agent use of gilteritinib in vitro displays greater anti-leukemic activity than quizartinib, when studied in oxygen conditions which mimic the hematopoietic niche (1% O2).
Read the full paper here: (subscription required): https://clincancerres.aacrjournals.org/content/early/2021/09/21/1078-0432.CCR-20-3114
Reduce Microbial Risk in a Closed Cell Production System through Controlled Temperature and Humidity
Congratulations to senior author Alicia Henn and team at BioSpherix on their work published June 1, 2021 in BioPharm International establishing that microbial bioburden is reduced in a closed cell manufacturing system through using the physical attributes of the atmosphere rather than toxic disinfectants.
Read the full paper here: https://www.biopharminternational.com/view/temperature-and-relative-humidity-control-to-reduce-bioburden-in-a-closed-cell-processing-and-production-system-without-disinfectants
BioSpherix Collaborates with Industry Leaders at the New RegeneratOR Test Bed to Advance Regenerative Medicine
Read the full press release: https://newscentral.site/regenerator-test-bed-to-launch-start-ups-advance-regenerative-medicine-ecosystem/
Establishment of an iPSC-based Preeclampsia Model with Trophoblasts and Hypoxia for Understanding Placental Dysfunction In Vitro
Congratulations to first author Mariko Horii and team at University of California San Diego on their work which was first published March 15 in Scientific Reports evidencing the utility of an in vitro model with iPSC-derived trophoblasts which were subjected to hypoxia (2% O2) for gaining human-relevant insight about preeclampsia and other placenta-based disorders during pregnancy.
Click here to access the journal article (open access): https://www.nature.com/articles/s41598-021-85230-5
Researchers Establish an In Vitro Model for Assessing Y90 Radiotherapy Effectiveness in Colon Cancer Cells at Tumor Relevant Oxygen Levels
Congratulations to first author Piotr Piasecki and colleagues at Military Institute of Medicine, Warsaw on their February 24 publication in Scientific Reports which demonstrated a cell-based model for evaluating anticancer activity by Yttrium-90 microspheres in colorectal cancer. Unlike most cell culture studies which occur at room air oxygen (21% O2), the team assessed antitumor activity at both physioxic (5% O2) and hypoxic (1% O2) conditions that replicate actual levels measured in solid tumors.
Click here to access the journal article (open access): https://www.nature.com/articles/s41598-021-84000-7
ITPP Attenuates Tumor Hypoxia In Vitro through Regulation of Angiogenesis and Enhances Anticancer Immunoresponse
Congratulations to lead author Bouchra El Hafny-Rahbi and colleagues from UPR 4301, Center for Molecular Biophysics, CNRS – Orleans on their findings first published online February 23rd in Journal of Cellular and Molecular Medicine which demonstrated in vitro that myo-inositol-trispyrophosphate (ITPP) modulates angiogenesis to reduce hypoxia in the cancer microenvironment and promote the antitumor immune response.
Click here to access the journal article (open access): https://onlinelibrary.wiley.com/doi/10.1111/jcmm.16399
Hypoxia Combined with Dynamic Compression In Vitro Promotes Matrix-Forming Phenotype in Meniscus Fibrochondrocytes
Congratulations to lead author Alexander Szojka and colleagues from University of Alberta on their findings first published February 6th in Journal of Tissue Engineering which demonstrated that combining physiologic oxygen and dynamic compression with fibrochondrocytes in vitro promotes phenotypic properties which are favorable for human engineered meniscus.
Click here to access the journal article (open access): https://journals.sagepub.com/doi/full/10.1177/2041731421990842
BioSpherix exceeds 3000 Customer Publications!
Click here to access the article: http://stemcellworkstation.net/pdf/Dec2020_Customer_Journal_Articles.pdf
Physiologically Relevant Oxygen In Vitro Unlocks New Understanding in Placental Research
Our thanks to Cytocentric Visionary – Dr. Sandeep Raha from McMaster University for sharing insight about the use of physiologic oxygen in a cell-based model of the maternal-fetal interface to enhance both the in vivo relevance and reproducibility of in vitro experiments.
Click here to watch the Interview : https://www.youtube.com/watch?v=1Z6FBeGZzGQ
Increased Penetration of Gold Nanoparticles in a Spheroid Blood-Brain Barrier Model following Hypoxic Exposure
Congratulations to lead author Viktoriya Sokolova and colleagues from University of Duisburg-Essen and Wake Forest School of Medicine on their findings published October 22nd in Scientific Reports demonstrating that ultrasmall gold nanoparticles can cross the blood-brain barrier and also that spheroid exposure to hypoxic levels increases BBB permeability and enhances nanoparticle penetration.
Click here to access the journal article (open access): https://www.nature.com/articles/s41598-020-75125-2
Stem Cell Webinar Oct 28th with PHI, SBI, and BioSpherix: Relevant Conditions. Relevant Answers.
Physiologic Oxygen Enhances the Neuroprotective Effect of Human MSCs
Congratulations to lead author Wioletta Lech and her colleagues from Mossakowski Medical Research Centre on their findings published October 9th in Scientific Reports which establish the significant effect physiological oxygen levels have upon the neuroprotective potential of human mesenchymal stem cells.
Click here to access the journal article (open access): https://www.nature.com/articles/s41598-020-74066-0
Cancer Research Webinar with PHI, SBI, and BioSpherix. Recording is now Available
If you missed our 1st webinar ‘RELEVANT CONDITIONS. RELEVANT ANSWERS. The Secrets to Truly Controlled Cell Experiments for Cancer Research’ Click Here