Metabolism / Mitochondria

biospherix metabolism

Aerobic metabolism is an efficient pathway for ATP production in mammalian cells. However, ‘standard’ cell culture methods include non-physiological exposures to both antibiotics and also room-air levels of oxygen. While convenient for preventing bacterial contamination, use of antibiotics in cell culture induces mitochondrial dysfunction and oxidative damage. While physiologic oxygen (physioxia) for most tissues is <5%, ‘normal’ exposure of room-air oxygen (21% O2) results in ROS artifact, altered cell function, and questionably relevant in vitro data.

Antibiotic-free, Physioxic conditions for cell culture:

  • reduce ROS artifact
  • facilitate metabolic reprogramming
  • regulate immunometabolism
  • improve mitochondrial morphology
  • modulate metabolic flux
  • control metabolic zonation
  • maintain the metabolome
  • reduce mitochondrial stress

New tools allow researchers to aseptically perform cell-based studies without antibiotics under physiologically relevant O2 conditions during all steps of cell incubation, cell handling, and cell analysis. Incubator subchambers upgrade the function of existing CO2 incubators. Cell-culture workstations provide full-time protection from contamination & room-air oxygen during the incubation, handling and analysis of cell cultures.