Metabolism / Mitochondria
Aerobic metabolism is an efficient pathway for ATP production in mammalian cells. However, ‘standard’ cell culture methods include non-physiological exposures to both antibiotics and also room-air levels of oxygen. While convenient for preventing bacterial contamination, use of antibiotics in cell culture induces mitochondrial dysfunction and oxidative damage. While physiologic oxygen (physioxia) for most tissues is <5%, ‘normal’ exposure of room-air oxygen (21% O2) results in ROS artifact, altered cell function, and questionably relevant in vitro data.
Antibiotic-free, Physioxic conditions for cell culture:
- reduce ROS artifact
- facilitate metabolic reprogramming
- regulate immunometabolism
- improve mitochondrial morphology
- modulate metabolic flux
- control metabolic zonation
- maintain the metabolome
- reduce mitochondrial stress
New tools allow researchers to aseptically perform cell-based studies without antibiotics under physiologically relevant O2 conditions during all steps of cell incubation, cell handling, and cell analysis. Incubator subchambers upgrade the function of existing CO2 incubators. Cell-culture workstations provide full-time protection from contamination & room-air oxygen during the incubation, handling and analysis of cell cultures.
Cell Research – aseptic, physiologic oxygen incubator and hood system, hypoxia workstation, hypoxia glove box with independently programmable control of O2, CO2, and Temperature in one (or more) modular incubator chamber(s) and/or cell handling chamber(s) - Xvivo System