Metabolism / Mitochondria

IMG 1041 editcrop

Aerobic metabolism is an efficient pathway for ATP production in mammalian cells. However, standard cell culture conditions (room air 21% O2) used in most experiments far exceed oxygen levels measured in healthy human tissues (physioxia < 5% O2). Routine cell exposure to supraphysiological oxygen results in mitochondrial ROS artifact, alterations in cell function, and questionable relevance of in vitro data.

Physioxic conditions for cell culture:

  • reduce ROS artifact
  • facilitate metabolic reprogramming
  • regulate immunometabolism
  • improve mitochondrial morphology
  • modulate metabolic flux
  • control metabolic zonation
  • maintain the metabolome
  • reduce mitochondrial stress

New tools allow researchers to simulate physiologically relevant O2 conditions during all steps of cell incubation, cell handling, and cell analysis. Oxygen-controlled subchambers upgrade the function of existing CO2 incubators. Physioxia workstations prevent brief disruptions of oxygen levels during the handling and analysis of cell cultures. Uninterrupted control of O2, CO2, Temp, and pH is a helpful strategy for adding physiologic relevance and enhanced reproducibility to cell-based research.