Closed incubators
and hoods for physiologically
relevant in vitro toxicology

Most labs aren’t equipped with tools for controlling oxygen. Room air conditions add ROS artifact to cell-based toxicological assays. This reduces the predictive capacity of tox assays, wasting precious time and money. Cell-based NAMs need protection from room air to accurately assess human risk.

Piossek F, et al. Physiological oxygen and co-culture with human fibroblasts facilitate in vivo-like properties in human renal proximal tubular epithelial cells. Chem Biol Interact. 2022 Jul 1;361:109959.

Bordt EA. The importance of controlling in vitro oxygen tension to accurately model in vivo neurophysiology. Neurotoxicology. 2018 May;66:213-220.

Coyle JP, et al. Reduced oxygen tension culturing conditionally alters toxicogenic response of differentiated H9c2 cardiomyoblasts to acrolein. Toxicol Mech Methods. 2018 Sep;28(7):488-498.

Nzou G, et al. Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling. Sci Rep. 2018 May 9;8(1):7413.

Cytocentric Blog – Interview with Cytocentric Visionary: Ian Mudway, Lecturer – Respiratory Toxicology Lung Biology Group , King’s College London

Part One: What Are the Fundamental Things That We Absolutely Have To Get Right?

Part Two: Taking a Career Risk for Better Cell Culture and Better Science

Part Three: We Will Know We Have Gotten Through When People Complain About the Difficulties of Publishing Data Generated under Atmospheric Oxygen