Blood / Hematology

biospherix hematology

Hypoxia/Hypercapnia prevents Cold-induced Injury of HSC/HPC during Storage.

Physiological oxygen values for human blood are far lower than those found in room air (21% O2). Arterial blood oxygen ranges 10-13% O2 and venous blood averages 5-6% O2. In healthy tissues, oxygen gradients are even lower. Bone marrow, where new blood cells originate, O2 levels can range from 6% down to 1% O2 (or less) depending on local vascular physiology. Since oxygen is critical in vivo for regulating cellular function, simulating body-like O2 in vitro adds physiological relevance to cell experiments.

Scientists upgrade CO2 incubators with O2-controlled subchambers that mimic physiologic O2 (or physioxia) during culture of lymphocytes (T cells and B cells), NK cells, macrophages, platelets, hematopoietic stem cells, hematopoietic progenitors, and leukemic cells.

Physioxia workstations permit incubation, handling, and analysis of cells under full-time optimal conditions (O2, CO2, Temp, and pH) to prevent cellular damage which occurs during even brief exposures to supraphysiological room-air oxygen.

For hypoxic and hypercapnic models (anemia, polycythemia, and beta thalassemia), choose hypoxia chambers, hypercapnia chambers, hypoxia gloveboxes, and hypoxia workstations to carry out highly reproducible O2/CO2 exposures in both cell and animal models.