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Cell Analysis

Controlled conditions are important for cell analysis. Today, most cell-based assays are performed in standard CO2 incubators, open BSCs, and room-air cell analyzers. Assay conditions in CO2 incubators vary greatly and never resemble the physiological cell environment. In spite of best practices, handling 96-well plates in open hoods exposes cells to inconsistent conditions, supraphysiologic O2, and subphysiologic Temp and CO2. These common laboratory practices followed by cell analysis in room air results in inconsistent data with poor scientific reproducibility, reduced translatability, and limited prediction value. The BioSpherix oxygen chambers and incubators offer more reliability during cell analysis. Variable, non-physiologic conditions for cell-based assays affect:

  • cell viability
  • metabolic function
  • immunophenotype
  • proliferation rate
  • cell morphology
  • protein production
  • cytotoxic response
  • migration activity
  • cytokine release
  • cell cycle

Cell Equipment

ProOx C21 & C-Chamber

Cell-Based Assays – physiologic oxygen chamber, hypoxia chamber with static control of O2 and CO2C21 & C-Chamber

Xvivo System

Cell-Based Assays – physiologic oxygen incubator and hood system, hypoxia workstation, hypoxia glove box with independently programmable control of O2, CO2, and Temperature in one (or more) modular incubator chamber(s) and/or cell handling chamber(s) – Xvivo System

Discover More


Cytocentric Blog – Interview with Cytocentric Visionaries: Sausan Jaber and Evan Bordt Do You Know? Oxygen and the Sex of Your Cultured Cells

Cytocentric Blog – Interview with Cytocentric Visionary: Shannon Mumenthaler, University of Southern California

Part One: Context is Everything, for Cells and for Scientists

Part Two: Oxygen, Cell Morphology, and Time

Cytocentric Blog – Interview with Cytocentric Visionary: Jan Jensen, Trailhead BioSystems

Part One: Unbiased Cell Science: Don’t Try to Out-Think Nature

Part Two: Controlling Stray Cell Fates in Culture

Part Three: Full-time Oxygen Control and Aseptic Conditions are Critical for Automated Protocol Optimization

Cytocentric Blog – Interview with Cytocentric Visionary: Ian Mudway, King’s College, UK

Part One: What Are the Fundamental Things That We Absolutely Have To Get Right?

Part Two: Taking a Career Risk for Better Cell Culture and Better Science

Part Three: We Will Know We Have Gotten Through When People Complain About the Difficulties of Publishing Data Generated under Atmospheric Oxygen

Cytocentric Blog – Interview with Cytocentric Visionary: Michael Gilkey, CEO Trailhead Biosystems

Part One: Optimizing Cell Media in 12 Dimensions: You Can’t Do This by Hand


Acea xCelligence



Seahorse XF


Oxygen Stress in Non-Physiologic Room Air

Supraphysioxic Room Air Adaption

Reducing plate edge effect by controlling cell handling conditions for
in vitro tumor hypoxia assays

Lab References

Cell Analysis at Centre for Innovative Medicine

Cell Analysis at Phase Holographic Imaging