Cancer / Tumor Biology
Hypoxia contributes to Cancer Aggressiveness
Hypoxia is a well known aspect of cancer biology. The Warburg Effect describes how tumor cells use metabolic pathways adapted to the low oxygen conditions (0-4% O2) in poorly vascularized, highly competitive tumor environments. Pathophysiologic hypoxia is a contributing factor in carcinogenesis, cell cycle, metabolism, angiogenesis, metastatic progression, induction of stem-like traits, immune evasion, and therapeutic resistance.
Hypoxia’s role in cancer cell phenotype is leading researchers to simulate the hypoxic cancer microenvironment in cell-based experiments to gain better understanding of tumors. Physiologically relevant oxygen environments improve the predictive capacity of cell-based assays. Cancer scientists Insert hypoxia chambers into existing CO2 incubators as an economical method to perform low oxygen (hypoxic) cell cultures.
Brief interruptions of hypoxia occurs during handling in open hoods. These disruptions alter the function of cells and are preveneted by hypoxic workstations (or glove boxes) which maintain constant control of O2 during all steps of cell incubation, cell manipulation, and cell analysis.
BioSpherix Ltd provides hypoxia chambers and hypoxia workstations designed to help cancer researchers increase both the reproducibility of experiments and the relevance of their work. The hypoxic chamber, ProOx C21 & C-Chamber is a subchamber system that allows researchers to add control of specific O2 levels within existing cell incubators. Add two or more ProOx C21 & C-Chamber hypoxia chambers to provide simultaneous control at multiple oxygen levels over the full range of pathophysiologic values.
The Xvivo System from BioSpherix Ltd is a hypoxia workstation that combines one or more O2 incubators with attachable closed hoods. This provides non-varying hypoxic conditions during the entire cell experiment. The Xvivo System may be outfitted with multiple O2 incubators so investigators can perform experiments at different O2 tensions at the same time. The Xvivo System’s modular hypoxia hoods are connected to the incubator chamber(s) to permit cell handling and cell analysis under the same conditions found in the incubator.