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Cytocentric® Incubator Subchamber

    Hypoxic Chamber for Cell Culture

  • Optimized for gas efficiency
  • Fast recovery time
  • Four standard sizes
  • No complicated “flushing” procedures
  • Magnetically sealed door – no latches!
  • Disassembles for easy cleaning and decontamination
  • Humidity pan provided
  • Hypoxia or Hyperoxia in vitro models
  • Physiologic oxygen (Physioxia) for cell culture
When you publish your data generated with this equipment, please copy and paste as follows for proper citation in your manuscript.

C-Chamber Overview


The C-Chamber is the foundation of the cytocentric subchamber cell culture system. It is functionalized by any of our gas controllers (for all bioactive gases, e.g. O2, CO2, CO, NO, etc.)


After the door is closed, our gas controller(s) will quickly restore the atmosphere inside the C-Chamber to your pre-programmed settings, minimizing the time your cells are exposed to room oxygen levels. If used with a cytocentric superchamber system, exposure to room oxygen levels is removed entirely.


The space-efficient design of the incubator subchamber for cell culture systems allows you to culture as few or as many plates as you require in precise conditions with minimal gas usage. Extreme oxygen levels consume large amounts of gas in a full incubator or glovebox chamber.


Chamber trays, water pan, guides and hardware are all made of 300 series stainless steel. The incubator subchamber for cell culture systems is made of clear polycarbonate and the door seal is magnetic. Temperature is controlled by the host incubator.


Our Hypoxia Chambers come stocked in the following sizes:


Part Number Description Size (Outside Dimensions)
C-174 1-shelf chamber 14”W x 12”D x 5.25”H
C-274 2-shelf chamber 14”W x 12”D x 6”H
C-374 3-shelf chamber 14”W x 12”D x 8”H
C-474 4-shelf chamber 14”W x 12”D x 10”H


Works in Every Incubator

The C-Chamber’s atmosphere can easily be controlled by any of our gas controllers including the ProOx (Oxygen Controller), ProCO2 (Carbon Dioxide Controller), OxyCycler profiling controllers (for all bioactive gases, e.g. Oxygen, Carbon Dioxide, Carbon Monoxide, Nitric Oxide, Ozone, etc.) and Xvivo system (bioactive gas and temperature control).

cchamber19 cchamber20 Hypoxia chambers have mounting cutouts for ProOx and/or ProCO2 sensor adapters or OxyCycler actuator pods or custom adapters for specialized needs. cchamber18 Each tray has a usable surface area for plates of 12.75” x 10.25” with a 2” clearance above the tray. After the door is closed, our gas controller(s) will quickly restore the atmosphere inside the C-Chamber to your pre-programmed settings, minimizing the time your cells are exposed to room oxygen levels.

Custom Cell Culture Chambers

BioSpherix has been working with leading edge scientists for 30 years developing custom cell culture chambers for a large variety of applications. We can build any chamber to suit your needs.

Installation Options

Single Setpoint Oxygen Controller 1 Chamber in CO2 incubator cchamber08 One controller requires mixed gas more affordable upfront Single Setpoint Oxygen & CO2 Controller 1 Chamber in Standard Incubator cchamber09 Using straight non-mixed gas, will save money in the long run 2 Single Setpoint Oxygen Controllers 2 Cell Culture Chambers in CO2 Incubator cchamber10 Expand your research one chamber at a time while saving space for standard experiments Oxycycler Dynamic Oxygen Controller 3 Chamber cchamber11 With 2 profiling chambers & 1 control hone in on your target much quicker Oxycycler Dynamic Oxygen & CO2 Controller 4 Chamber cchamber12 With 4 Profiles, expedite results with less work

Gas Consumption

With 4 sizes of culture chambers you can truly customize your equipment to meet your research. Gas efficient cell culture has never been easier. Combine with a gas controller and you will no longer return expensive half-used premixed gas cylinders. Our chambers range from around 7-15% total volume of a typical benchtop incubator. 85-90% less volume can lead to a significant gas savings. cchamber13

Avoid Sub-Optimal Conditions

Most cells in your body are ~3% O2. Unfortunately most scientists culture cells in room air which is 85% higher than typical physiologic conditions. Oxygen has been shown to be toxic at high and low levels. In today’s world, elevated costs of operation and increased competition make it more important than ever to get consistent early-verifiable results. Every time your incubator door is opened and your cells start to see inconsistent conditions this can increase the noise in most experiments. C-Chambers can help reduce the signal-to-noise ratio. C-Chambers will also decrease recovery times dramatically; the graph below displays conditions in an incubator throughout a typical 12 hour workday. The 30 minute recovery times and non-optimal exposures are all but eliminated in a C-Chamber system cchamber14


Sometimes cells need to be transported down the hall, around the lab, or even to adjacent buildings for the next step in their research. With a C-Chamber you can just unplug and go. The C-Chamber will ensure your cells are protected from sub-optimal conditions at all times. cchamber15


Some experiments require cells be removed from the incubator for a great amount of time, whether you have a long distance to go, or your cells need preconditioning according to your protocol the C-Chamber can provide the right environment. Oxygen sensitive research is often corrupted when cells are exposed to sub-optimal conditions; random intervals each time a process is completed greatly magnifies the problem. cchamber16


Up to four experiments in a single incubator. Never waste valuable incubator space with a specific protocol. Combine a few C-Chambers with our multi-chamber gas controllers and accelerate your research. cchamber17