Blood / Hematology

biospherix hematology

Hypoxia/Hypercapnia prevents Cold-induced Injury of HSC/HPC during Storage.

Learn how BioSpherix equipment can help increase experimental reproducibility in blood research below.

Physiological oxygen values for human blood are far lower than those found in room air (21% O2). Arterial blood oxygen ranges 10-13% O2 and venous blood averages 5-6% O2. In healthy tissues, oxygen gradients are even lower. Bone marrow, where new blood cells originate, O2 levels can range from 6% down to 1% O2 (or less) depending on local vascular physiology. Since oxygen is critical in vivo for regulating cellular function, simulating body-like O2 in vitro adds physiological relevance to cell experiments.

Scientists upgrade CO2 incubators with O2-controlled subchambers that mimic physiologic O2 (or physioxia) during culture of lymphocytes (T cells and B cells), NK cells, macrophages, platelets, hematopoietic stem cells, hematopoietic progenitors, and leukemic cells.

Physioxia workstations permit incubation, handling, and analysis of cells under full-time optimal conditions (O2, CO2, Temp, and pH) to prevent cellular damage which occurs during even brief exposures to supraphysiological room-air oxygen.

For hypoxic and hypercapnic models (anemia, polycythemia, and beta thalassemia), choose hypoxia chambers, hypercapnia chambers, hypoxia gloveboxes, and hypoxia workstations to carry out highly reproducible O2/CO2 exposures in both cell and animal models.