bp09 headerThe in vivo Relevance of Past Studies Performed in Room Air

A recent paper in Cell (paywalled) from the Broxmeyer group at Indiana University coined the term extraphysiologic shock/stress (EPHOSS) to describe a distinct negative effect of exposure to room air on hematopoietic stem cell (HSC) yields. [1] In this study, the authors isolated mouse bone marrow HSC with one femur in an atmosphere of constant 3% oxygen and the other in room air. They also took an aliquot of bone marrow cells protected from room air during isolation and exposed it to ambient air during processing for comparison. Learn how in vivo research is affected by room air temperature in our post below.

bp10 headerThe Reproducibility Crisis and the CytoCentric Environment

There has been a lot of public attention directed at scientific reproducibility, most recently in articles in PLOS Biology, and in the Washington Post. A National Academy of Sciences workshop, and a University College London event, have also focused public attention on the etiology of irreproducible biomedical studies. The inability to get the same results in study after study has tremendous economic and social impact on our country. Continue reading our post below to learn why a cell focus in research is so critical and how cytocentricity helps scientific reproducibility.

bp8 01How “Closed” is a Functionally Closed System?

Open. Closed. Functionally Closed. What do these terms mean in bioprocessing? Learn about the purpose of functionally closed systems in bioprocessing below.

Clear definitions were published in a classic BioPharm International article[1] here, in part:

Cells Need Protocol

bp06 image002There are many ways to use an incubator to grow cells. Many laboratories use the same incubator for many different protocols at the same time. In a perfect lab, the cells in each protocol would stay in their own incubator and nobody else would open the door after they go in. Due to logistics, though, most laboratory incubators look more like the one above. Learn more about the advantages of cell protocols in lab research below.

Whose turn was it to check the water pan this week?

It is impossible to provide unbroken, optimized conditions for your cells in a shared incubator. The more an incubator is shared, the more your cells are at risk.

Cells Need Dynamics

bp05 image001Learn the reasons dynamic oxygen controls matter in the article below.

The cells in the two panels above are the same cultured K562 cells in photomicrographs taken hours apart – except that the cells aren’t really the same, are they?

Living cells just don’t stay the same over time. In fact, central to the very definition of “life” is the ability to change and grow.

Looking at the cells above from their point of view, three out of the four original cells have clearly divided, so their needs in culture have now changed. The cells, as a population, need more nutrients, more oxygen, and they need more wastes removed. In a few days, they will be subcultured to keep them growing in what would otherwise have been suboptimal conditions. We can anticipate that cells will have these needs, so we plan to subculture them.