The Biology of HIF Proteins Impacts the Outcome of Your Experiments in Physiologic Oxygen:

Considerations for protocol design

We recently posted an article “What Oxygen Level Should I Use for My Cells In Vitro?” in response to questions that we have fielded from customers getting started in cytocentric research. To expand on that topic, we are posting here a few more resources for information on the mechanism of how changes in oxygen levels impact cells in culture.

Mesenchymal Stem Cells for Cellular Therapies and Regenerative Medicine: 

Cytocentric oxygen control makes sense from the molecular level through to the bottom line.

Mesenchymal stem cells (MSC) have shown tremendous promise as a cellular therapy for indications as varied as arthritis [1], diabetes [2], cardiac disease [3], wound repair [4], graft-versus-host disease [5], ALS [6], spinal cord injury [7], even infectious disease [89]. MSC also have the potential to transdifferentiate into multiple cell lineages for regenerative medicine applications [10]. But for MSC to live up to that promise, the best conditions for expanding and studying these critical cells in the laboratory must be established.

What Oxygen Level Should I Use for My Cells In Vitro?

Well, it depends.

Learning how to determine oxygen levels for your in vitro culture is important to producing relevant results.

Are you looking for physiological relevance or comparison with cells cultured in a standard room-air incubator?

First, you should know that there is nothing normal or “normoxic” about room air oxygen for cells that normally reside inside the body.In vivo, as soon as air enters the body, it mixes with CO2 being expired and the percentage of oxygen decreases.

22 headerBiocontainment: Cells Need Technician Protection

“I’ve been handling cells this way for years and I’VE never gotten sick.”

How many times have you heard that, and doubted it?

Continue reading to learn why biosafety and biocontainment have become increasingly important.

Confidence in handling potentially dangerous cells, tissues, and vectors, can easily become complacency. Now in an uncertain era of CRISPR/Cas GMOs and gene drives, biocontainment and biosafety have taken on more urgency.

In Vitro Reproducibility and Experimental Conditions:

Is There an Elephant in the Room Air?

If another lab tried to reproduce your results, would they fail?

It’s a scary thought.

Amongst the recent publications on the Crisis in Reproducibility, there has been extensive discussion of the importance of factors from raw materials through published reports. These include; improper statistics [1], experimental design, controls, reagent validation, and reference standards [2], cell line misidentification [3], animal cage environment [4], raw data availability [5], and data reporting [6].

Beyond equipment validation, only one author has addressed variability in the in vitro cell microenvironment, arguing that it is one factor that makes the push for replication unwise. [7]