We recently posted an article “What Oxygen Level Should I Use for My Cells In Vitro?” in response to questions that we have fielded from customers getting started in cytocentric research. To expand on that topic, we are posting here a few more resources for information on the mechanism of how changes in oxygen levels impact cells in culture.
Mesenchymal stem cells (MSC) have shown tremendous promise as a cellular therapy for indications as varied as arthritis , diabetes , cardiac disease , wound repair , graft-versus-host disease , ALS , spinal cord injury , even infectious disease [8, 9]. MSC also have the potential to transdifferentiate into multiple cell lineages for regenerative medicine applications . But for MSC to live up to that promise, the best conditions for expanding and studying these critical cells in the laboratory must be established.
Well, it depends.
Learning how to determine oxygen levels for your in vitro culture is important to producing relevant results.
Are you looking for physiological relevance or comparison with cells cultured in a standard room-air incubator?
First, you should know that there is nothing normal or “normoxic” about room air oxygen for cells that normally reside inside the body.In vivo, as soon as air enters the body, it mixes with CO2 being expired and the percentage of oxygen decreases.
“I’ve been handling cells this way for years and I’VE never gotten sick.”
How many times have you heard that, and doubted it?
Continue reading to learn why biosafety and biocontainment have become increasingly important.
Confidence in handling potentially dangerous cells, tissues, and vectors, can easily become complacency. Now in an uncertain era of CRISPR/Cas GMOs and gene drives, biocontainment and biosafety have taken on more urgency.
If another lab tried to reproduce your results, would they fail?
It’s a scary thought.
Amongst the recent publications on the Crisis in Reproducibility, there has been extensive discussion of the importance of factors from raw materials through published reports. These include; improper statistics , experimental design, controls, reagent validation, and reference standards , cell line misidentification , animal cage environment , raw data availability , and data reporting .