Cytocentric-Blog

bp042 image01What is the O2 Level in My Cell Culture Incubator?

(Hint: It’s Not 21%)

Because room air is assumed to be near 21% oxygen, researchers often report that their room air incubator is as well. However, if you would think about it for a minute, you would realize that this is impossible.

 We charge a traditional open room cell culture incubator with CO, raising it to 5% or more, to maintain the pH of carbonate buffer-based cell culture media. Room air contains only 0.03 - 0.06% CO2 (or sometimes a little more if your ventilation is poor). Every time the inner incubator door is opened and closed, CO2 is infused to replace what is lost to the room. This necessarily drives the percentage of oxygen down inside the incubator.

cytocentric visionaries mg p3Cytocentric Visionaries: Michael Gilkey, CEO Trailhead Biosystems

Part Three: Let the Cells Lead the Way through the Matrix and Don’t Be Afraid of Change.

In part 2, we sit with Trailhead Systems CEO, Michael Gilkey, to discuss using the Xvivo system to research different cell types.

Here, we continue the conversation, talking about the leap that researchers have to take to leave their old way of doing things and invest in a system defined by the cells. This conversation was edited for length. Read our interview transcripts to learn why Trailhead Biosystems is focusing on cell-centered research.

cytocentric visionaries mg p2Cytocentric Visionaries: Michael Gilkey, CEO Trailhead Biosystems

Part Two: How to Get that Just-Right Cell in the Middle

Alicia Henn, Chief Scientific Officer, BioSpherix

IIn the first part of our interview with Michael Gilkey, CEO of Trailhead Biosystems, we discuss optimizing cell therapy media with physiologic conditions as well as the power of Trailhead’s unique combination of robotics, Design of Experiments-based methods, and mathematical modeling to identify the best combinations of reagents for recapitulating cell development in vitro.

Here, we continue the conversation, talking about all the different cell types Trailhead is addressing with their unique platform. Continue reading to learn how Trailhead Biosystems is using the Xvivo System to research different cell types.

cytocentric visionaries mg p1Cytocentric Visionaries: Michael Gilkey, CEO Trailhead Biosystems

Part One: Optimizing Cell Media in 12 Dimensions: You Can’t Do This by Hand

Alicia Henn, Chief Scientific Officer, BioSpherix

In addition to being CEO of the Ohio start-up Trailhead Biosystems, Michael Gilkey is the Director of Strategic Partnerships for the National Center for Regenerative Medicine. Michael and his team have established a unique platform for Design of Experiments Theory-based cell differentiation optimization. Trailhead uses an Xvivo barrier isolator system from BioSpherix to protect the cells from room air and maintain physiologic oxygen levels

Here, Alicia talks with Michael about using a 12-dimension hypercube of variables, robotics, and mathematical modeling to quickly identify the best combinations of components for media used in cellular therapeutics. This conversation with Dr. Michael Gilkey to discuss optimizing cell therapy media with physiologic conditions was edited for length.

cytocentric visionaries dp p3Cytocentric Visionaries: Donald Phinney

Part 3: The Cell Cycle Is the Redox Cycle, and All Clinical MSC are Stressed

In Part Two, Chief Scientific Officer Alicia Henn talked with Dr. Phinney about MSC, the exosomes that they produce and therapeutics. Today we talk about MSC and oxidative stress in vitro. This conversation on the importance of studying the cellular redox balance in MSC cells was edited for length.