Cytocentric-Blog

cytocentric visionaries im p2Cytocentric Visionaries: Ian Mudway, King's College, UK

Part Two: Taking a Career Risk for Better Cell Culture and Better Science

In Part One, Dr. Henn talked with Dr. Ian Mudway about his recent publication , “Quantifying the magnitude of the oxygen artefact inherent in culturing airway cells under atmospheric oxygen versus physiological levels.”

Here, they discuss barriers to change in the scientific community.

cytocentric visionaries im p1Cytocentric Visionaries: Ian Mudway, King's College, UK

Part One: What Are the Fundamental Things That We Absolutely Have To Get Right?

Ian Mudway is a Lecturer in Respiratory Toxicology at King’s College in London. He has been active in air pollution research, participating in Air Quality conferences, speaking out on BBC World News and in the Guardian newspaper, and advising the World Health Organization.

Here, Dr. Henn talks with Dr. Mudway about his recent publication , Quantifying the magnitude of the oxygen artefact inherent in culturing airway cells under atmospheric oxygen versus physiological levels.”

Mini-Review: Cord Blood HSC and Physiologic Oxygen

At what oxygen level should I culture my umbilical cord-derived hematopoietic stem cells? It’s not a question you will hear on Jeopardy, but it is a critically important question.

Hematopoietic stem cells from umbilical cord blood (CB-HSC) have been shown to be beneficial for treating leukemia1,2, neuroblastoma3, and is of great interest for other disease applications. NIH-funded clinical trials listed on clinicaltrials.gov include studies of CB-HSC for immunodeficiencies, autoimmune diseases, sickle cell, cerebral palsy, complications of prematurity, and stroke. However, individual cord blood units often do not have the cell yield needed for a full transplant, requiring the pairing of HLA- partially matched cord blood units for a single transplant4. This presents obvious drawbacks in limiting the numbers of patients that can be treated.

bp042 image01What is the O2 Level in My Cell Culture Incubator?

(Hint: It’s Not 21%)

Because room air is assumed to be near 21% oxygen, researchers often report that their room air incubator is as well. However, if you would think about it for a minute, you would realize that this is impossible.

 We charge a traditional open room cell culture incubator with CO, raising it to 5% or more, to maintain the pH of carbonate buffer-based cell culture media. Room air contains only 0.03 - 0.06% CO2 (or sometimes a little more if your ventilation is poor). Every time the inner incubator door is opened and closed, CO2 is infused to replace what is lost to the room. This necessarily drives the percentage of oxygen down inside the incubator.

cytocentric visionaries mg p3Cytocentric Visionaries: Michael Gilkey, CEO Trailhead Biosystems

Part Three: Let the Cells Lead the Way through the Matrix and Don’t Be Afraid of Change.

In Part Two, we talked to Michael Gilkey, CEO of Trailhead Biosystems about the many different projects they have planned.

Here, we continue the conversation, talking about the leap that researchers have to take to leave their old way of doing things and invest in a system defined by the cells. This conversation was edited for length.