Why Optimize MSC Cell Culture Conditions?

Feed a Fibroblast, Starve a Stem Cell

A new publication in Stem Cell Research & Therapy (open access) from Alan Wells’ group at University of Pittsburgh has some highly relevant findings for Mesenchymal stem/stromal cell (MSC) culture optimization. MSC are an incredibly valuable cell type for both industrial and research scale cell-based therapeutics. The Wells group reported that in their investigations of the relationships between MSC starvation, autophagy, and differentiation, they stumbled upon a strikingly high glucose consumption rate in comparison with other cell types.1

cytocentric visionaries sm p2Cytocentric Visionaries: Shannon Mumenthaler, University of Southern California

Part Two: Oxygen, Cell Morphology, and Time

In Part One of this two-part interview, we talked with Dr. Shannon Mumenthaler about her latest studypublished in Nature’s Open Access Journal, Scientific Reports [1] and her unique combination of high throughput image analysis, heterogeneous cell culture, and full-time control of conditions. Today we talk about adding new dimensions into highly dimensional cell parameter space, including cell shape and time. Read the full interview transcripts below to learn more about how cell morphology can be used to better understand cancer biology.

cytocentric visionaries sm p1Cytocentric Visionaries: Shannon Mumenthaler, University of Southern California

Part One: Context is Everything, for Cells and for Scientists

Dr. Shannon Mumenthaler is an Assistant Professor of Research Medicine and Lab Director of the Lawrence J. Ellison Institute for Transformative Medicine of USC. She works closely with a multidisciplinary team, including mathematicians for data analysis. Her group’s latest study published in Nature’s Scientific Reports [1] describes a unique technological combination of high-throughput cell imaging with physiologically relevant oxygen levels in a cytocentric barrier isolator.

In Part One of this two-part interview, we talk with Dr. Mumenthaler about her approach for acquiring a better understanding of the complex drug responses of tumors in their natural environment. Read the full transcripts of this interview to learn more about the importance of controlling oxygen in the tumor microenvironment.

hypoxia cell growth biospherix labLonza's CytoSMART Lab for Cell Growth under Hypoxia

Full-time Control of Conditions during Cell Handling Enhances the Expansion of Human MSC for Stem Cell Therapies

Research Interest

BioSpherix's scientific laboratory explores Cytocentric™ environments, in vitro spaces designed so that cells are protected from room air at all times, even during cell handling. A controlled environment is essential for good science, yet the conventional open room laboratory provides highly unstable and non-physiologic conditions for cells.

cytocentric visionaries hb p1Cytocentric Visionaries: Hal Broxmeyer, Indiana University

How the Shock of Room Air Oxygen Reduces Stem Cell Yields

Dr. Hal Broxmeyer is a Distinguished Professor, Professor of Microbiology/Immunology and Co-Leader of the Program on Hematopoiesis, Malignant Hematology, and Immunology at Indiana University Simon Cancer Center. He has chalked up well over 1,000 scientific publications and his work has been seminal to the field of hematopoietic stem cell transplantation. His group’s 2015 paper in Cell and the subsequent review articleare landmark papers that demonstrate how room air oxygen exposure during cell isolation inflicts damage on freshly isolated stem cells.

Here, we talk with Dr. Broxmeyer about his work and learn how relevant oxygen conditions affect hematopoietic progenitor cells. This interview was conducted by email and edited for length.