Going the extra mile to meet the needs of cells can pay big dividends. Conventional cell culture labs accept and even expect a surprising amount of variability, inconsistent results, and setbacks caused by failed experiments & contamination.
Cytocentricity will save you time and money by decreasing variability, increasing consistency, avoiding contaminations, and reducing the number of failed experiments. Best of all, it will make your data more relevant, and thus fortify the credibility of your conclusions.
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The Earth Is Flat...
The Sun Revolves Around The Earth...
Oxygen Is Not Important In Cell Culture...
Data is the backbone of your research. When your data is generated from cells under conditions different from what they would normally experience in the body, relevance of that data is questionable. By better simulating physiologic conditions, Cytocentricity will give you a truer picture of what is real. Your hypotheses will be more accurate. Your conclusions will be hardier and more likely to withstand the tests of time and scrutiny. If your research is respected, funding and career opportunities are larger. Irrelevant data makes your research and even your career a house-of-cards. Don't take the chance. It may eventually crumble.
In the old days, cell culture was all about cell growth. Now it is more about the induction or maintenance of phenotype. Cytocentricity gives you unprecedented control over phenotype. First, more critical cell parameters that affect phenotype are offered (O2, CO, NO, etc.). Second, comprehensive control of those parameters is inherent. Phenotypic response to an environmental cue is sensitive, so finesse is the key. Dynamic control provides you with patterning to deliberately induce a change in gene expression. Uninterruptible control of all parameters prevents unintended confusions in gene expression to better maintain phenotype. Newly recognized fundamental role of O2 in gene expression may impact all cells. Nothing does O2 better than our equipment designed for oxygen..
DECREASE EXPERIMENTAL VARIABILITY
The cost of variability is hidden but can be huge. High variability manifests as a large standard deviation. Good experimental design (95% confidence?) with a large standard deviation requires many more experimental points, many more samples. Cytocentricity, or better attention to cell needs, will decrease experimental variability, decrease your standard deviation, and thus reduce the amount of materials (fewer plates, less media, etc.) and decrease the labor and overhead required to perform the experiment with no compromise in your confidence level! And tighter data gives all your cell assays better resolution, more power to detect subtle differences.
What to do with inconsistent results ? ...with data that doesn't fit ? ...with data that doesn't make sense ? Do you throw it out ? Do you throw out the whole experiment? If so, you have a total loss. All the material, all the work, and all the overhead that it took to do the experiments are wasted. What causes inconsistent results? One cause is human error. For example, processing cells in open hoods exposes cells to suboptimal conditions, and everybody sees it. The media turns pink. So we hurry. SOPs get sloppy. The result is inconsistent data. Cytocentricity will make your results more consistent. Cytocentric processing in closed incubated hoods avoids the suboptimal conditions, avoids the need to hurry, decreases human sloppiness, and increases consistency.
Cell culture labs expend a major effort to avoid microbial contamination. Yet, despite the effort, most labs not only accept it but expect it, and do experience contaminations on a routine basis. The cost of lost experiments and cost of decontamination in lost productivity is significant, and can be absolutely huge. Cytocentricity can reduce contamination risk by large factor.