What’s New
SUNY Alfred Students visit BioSpherix
Thanks to Dr. Steve Bauer and his class at SUNY Alfred for driving all the way to Parish, NY to visit us! We had a blast mimicking a cell production process in the Xvivo System. Each of these bright young people would make a marvelous addition to the Regenerative Medicine workforce, bringing the newest cell and tissue therapies to the patients that need them. They are poised to bring about the future of biotech.
Click here to read more: https://www.alfredstate.edu/news/2023-05-16/biotechniques-class-visits-biospherix
Our CSO, Alicia Henn, to Moderate Upcoming NIST Workshop on Measurement in Biofabrication
Biofabricated tissue-engineered constructs have the potential to transform personalized medicine. However, characterizing these constructs post-fabrication and throughout preclinical use remains challenging. We will hold a 1-day workshop on measurement needs for biofabricated constructs that contain cells, focusing on metrology for the structure of the constructs, cell viability in the constructs, and functional capacity of the constructs.
Click here to read more: https://www.nist.gov/news-events/events/2022/12/workshop-measurement-needs-biofabrication-tissue-engineered-medical
Enhancing Clinical Translation with Preclinical Physioxia
Professors Adebayo and Nakshatri review the effect of extraphysiological oxygen shock/stress on cancer cells, even when they are exposed to ambient air only briefly.
Click here to read more: https://pubmed.ncbi.nlm.nih.gov/36169928/
Hypoxia Enhances NMJ Formation
Reprogrammed myoblasts improve the formation of neuromuscular junctions in this pioneer study by Tolsma et al.
https://pubmed.ncbi.nlm.nih.gov/36208481/
Congratulations to Emma Warner a WFIRM 2022 Scholar, who presented her data at the BMES Annual Meeting.
Emma used the Xvivo System Model X2 in her work at the RegMed TestBed at WFIRM this past summer.
Click here to read more: https://www.pharmamirror.com/pharmaceutical-articles/testing-facility-regenerative-medicine/
Neuroinflammation Regulation Therapies Tested Using Hypoxia
Wang et al. recreate pathophysiological conditions to study neuroprotection after cardiac arrest-induced brain injury.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35921049/
Intestinal Wound Healing and HIF
Steiner et al. review the double-edged sword HIF presents over the course of the metabolic response to chronic intestinal inflammation.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35912990/
Identifying EV Uptake Signals in Aggressive Pancreatic Cancer
Nigri et al. mimic pathophysiologic conditions to identify a potential target for therapeutic intervention into aggressive pancreatic ductal carcinoma.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35917363/
Osteogenesis and HIF Signaling in the Clinic
An in-depth review of recent progress in HIF-regulated bone regeneration provides evidence for novel tissue repair strategies.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35913258/
Creating Relevant Trophoblast Pathophysiology in vitro
Cheng et al. control oxygen around primary human trophoblasts to study autophagy-lysosomal machinery in early-onset preeclampsia.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35628454/
Relevant miRNA Patterns Under Pathophysioxia
Majewska et al. study the role of PTEN in kidney cancer by mimicking the TME in vitro.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35625614/
Extracellular Vesicles Require Proper Conditions
Almeria et al. detail how MSC produce more heterogeneous EVs when the upstream culture conditions are uncontrolled.
Click here to read more (open access): https://pubmed.ncbi.nlm.nih.gov/35501833/
Improving Tox Assay Predictability with Physioxia
Human renal proximal tubular epithelial cells exhibit in vivo-like properties in co-culture under physioxia, Piossek et al. report.
read more: https://pubmed.ncbi.nlm.nih.gov/35533734/
Oxygen Controls the Lymphovascular-Immune Ensemble
Schito et al. review the role of hypoxia in the lymphatic system’s response to cancer.
read more: https://www.cell.com/trends/cancer/fulltext/S2405-8033(22)00095-4?
Physioxia Influences Gene Methylation
Dogan et al. use physiological oxygen to examine epigenetic modification in pluripotent stem cells.
Click here to read more (open access): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148100/
Altering O2 in Solid Tumor Studies
Herrera-Campos et al. review the role of hyperoxia and the immune system in novel treatments for solid tumors.
Click here to read more (open access): https://www.mdpi.com/2072-6694/14/11/2740/htm
Enhanced Self-Renewal of HSC Under Physioxia
Physioxia induces downregulation of Tet2 in Hematopoietic Stem Cells, report Aljoufi et al.
Click here to read more (open access): https://ashpublications.org/blood/article/doi/10.1182/blood.2022015499/485747/Physioxia-induced-Downregulation-of-Tet2-in
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Recent News
Modernizing therapeutic cell production
Phase Holographic Imaging (PHI) visits the Wake Forest Institute for Regenerative Medicine (WFIRM) to finalize an agreement concerning the automation of advanced quality control in large-scale biomanufacturing of regenerative therapies.
trending
Testing Facility Called “Game Changer” in Regenerative Medicine. The RegeneratOR Test Bed is an exciting game changer. The testing facility, located in North Carolina, provides supportive technology at no cost allowing companies to test and prove their cell-based products at various stages of development.
recent publications
Labuz, D. F., et al. Intrauterine Growth Restriction (IUGR) as a potential target for transamniotic stem cell therapy Journal of pediatric surgery. 2022; doi:10.1016/j.jpedsurg.2022.01.062
Ozgür B, et al. Hypoxia increases expression of selected blood-brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers. Fluids Barriers CNS. 2022;19(1):1. doi:10.1186/s12987-021-00297-6
Cahill, L. S., et al. Sex differences in modulation of fetoplacental vascular resistance in growth-restricted mouse fetuses following betamethasone administration: comparisons with human fetuses
Am J Obstet Gynecol MFM doi:10.1016/j.ajogmf.2020.100251 (2021).
Cluff, E., et al. Hypoxia-inducible factor-1 alpha expression is induced by IL-2 via the PI3K/mTOR pathway in hypoxic NK cells and supports effector functions in NKL cells and ex vivo expanded NK cells. Cancer Immunology, Immunotherapy 2022; doi:10.1007/s00262-021-03126-9
from the blog
Modeling Acute Kidney Injury
Kim et al. discover a recombinant protein cocktail that accelerates kidney repair after renal ischemia-reperfusion injury.
read more: https://www.frontiersin.org/articles/10.3389/fbioe.2022.848679/full
Regulators of Cholesterol Homeostasis in Human Placental Endothelium
Strahlhofer-Augsten et al. dive into the role of SR-BI in vasculature by mimicking the placental environment with physioxia.
read more: https://pubmed.ncbi.nlm.nih.gov/35628180/
Reproducing Ovarian Tumor Conditions in vitro
Strahlhofer-Augsten et al. dive into the role of SR-BI in vasculature by mimicking the placental environment with physioxia.
read more: https://www.nature.com/articles/s42003-022-03495-6
Oxygen Regulates Tumor Immune Escape
Wu et al. review the role of HIF in TME regulation.
read more: https://jhoonline.biomedcentral.com/articles/10.1186/s13045-022-01292-6
“Maintaining Physioxia in Cell Culture is Imperative”
Alva et al. review the current state of supraphysioxia in mammalian cell culture and call for better practices.
read more: https://www.mdpi.com/2073-4409/11/19/3123
Modeling OGD with a Controlled Environment
Calabrese et al. study resilience to neuronal ischemic injury by modeling oxygen glucose deprivation with an oxygen-controlled processing chamber.
read more: https://pubmed.ncbi.nlm.nih.gov/36229429/