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OxyCycler A44C

Dynamic CO2 Controller for Animal Modeling

  • Static or Dynamic CO2 control
  • Reproducible Hypercapnia Exposures
  • Controls four chambers independently
  • Monitoring and quality control
  • Data logging
  • Convenient PC user interface
  • Hypercapnia In Vivo
When you publish your data generated with this equipment, please copy and paste as follows for proper citation in your manuscript.

OxyCycler A44C Overview


The OxyCycler A44C is a powerful research tool for scientists who do CO2 sensitive work. It makes complex CO2 profile control easy.


Use this dynamic CO2 controller for animal modeling to control carbon dioxide profiles with multiple setpoints anywhere from 0.1-20%.

Hold any setpoint for any length of time. Rate of change between any two setpoints is fully adjustable. Cycle any profile any fixed number of times, or continuously.


The OxyCycler A44C works exceptionally well with BioSpherix A-Chambers. However, it can work with practically any semi-sealable enclosure. Large or small. Square or round. Flexible or rigid. Manufactured or custom made. Most chambers can be fitted in minutes.


The dynamic CO2 controller for animal modeling controls up to four chambers at once. Operate all chambers simultaneously, or each independent of the others. Control different profiles, or same profile in every chamber. Start all at the same time, or each at different times.


Nitrogen or compressed dry air is infused to lower CO2. Carbon Dioxide is infused to raise it. Feedback from the carbon dioxide sensor inside chamber tells the OxyCycler A44C exactly infuse. No gas is ever wasted! Any disturbances are immediately detected and corrected.


Once installed and configured, it’s easy. Check calibration once in a while and don’t run out of gas. Otherwise it’s all automatic! Can work continuously year round, or on occasion as needed.




CONTROL RANGE: 0.1-20% Carbon Dioxide



GAS SOURCE: Compressed gas tanks, liquid carboys (from headspace), or generators.

GAS SUPPLY: Pressurized CO2, N2.

GAS SUPPLY LINE: 1/4 inch I.D. hose pressure rated at 40 PSIG.


GAS INFUSION RATE: 1-150 S.C.F.H. each control gas each chamber.

GAS SUPPLY HOSE FITTINGS: 1/4 inch hose barb.

UMBILICAL LENGTH: 12 feet (custom lengths available).

ACTUATOR POD SIZE: 7”H, 4.375”W x 4.5”D inches.

ALARM OUTPUT: Visible flashing indicator. PC adds audible and more visible indicators.

ALARM MODES: Process high, process low, deviation high, deviation low, deviation band.

WEIGHT: 31 lbs. (Controller Only)

DIMENSIONS: 9”H x 22.2”W x 18.5”D

Sensor Operational Parameters

HOST CHAMBER TEMPERATURE: 0-40°C (depending on sensor).

HOST CHAMBER CO2: 0-20% (depending on sensor).

HOST CHAMBER HUMIDITY: 1-95% R.H. (depending on sensor).


Front Panel


1. Controller: Bright blue digits on black back ground. Continuously displays current control gas level, control status, and alarm status in all chambers. Displays menu items and settings during programming.

2. Bleed Valves and Barbs: Bleeds gases out of gas supply lines. Calibration cup for sensor attaches here.

3. ZERO Cal Gas Flowmeter: Used for calibration.

4. SPAN Cal Gas Flowmeter: Used for calibration.

5. Needle Valves: Sets infusion rate of control gases in each chamber to accommodate different dynamics. Can manually override controller to shut off gas.


Back Panel


6. Power Receptacle: 12VDC power supply connects here.

7. Actuator Pod Umbilical: Flexible umbilicals connect remote actuator pods to back panel. Semi-swivel connectors at both ends allow 360° orientation. Some models are hard welded; function is the same.

8. Alarm Receptacle: Connect an appropriate alarm to this jack.

9. RS 485 Connections: One cable attaches to a computer and the other cable attaches to another unit, to allow communication with the computer (if applicable).

10. Supply Gas Hose Barb: Barbs for 1/4 inch I.D. hose from gas sources. Handles pressure up to 40 PSIG.

11. Ground Stud: For grounding the unit to protect from electric damage.




1. Set OxyCycler A44C on or near host chambers and plug it in.

2. Mount each actuator pod to its host chamber.

3. Hook up gas supply.



Use PC software for easy interface, real-time trend charting, data logging, and remote operation. Dynamic CO2 exposures are programmed with a series of setpoints that can change, be stored and re-run with the click of a mouse.

How It Works


From outside four chambers, the OxyCycler A44C works by remotely sensing carbon dioxide inside each chamber, infusing compressed dry air or nitrogen to reduce carbon dioxide, and infusing carbon dioxide to raise it. A continuous supply of both up and down gases are required. Compressed dry air vs nitrogen will depend upon your setup and protocol.

The OxyCycler A44C connects to the chambers via four flexible umbilicals. At the tip of each umbilical is an actuator pod which contains a carbon dioxide sensor, a gas nozzle, and mounting hardware. Pods mount to chamber over special precut holes so CO2 sensors can monitor chamber carbon dioxide and gas can be infused.



Conveniently utilizes gas from any source. Compressed gas is best in low consumption applications. Generator is best in high consumption applications. Liquid is best in between.


Maximum efficiency reduces chamber gas consumption.


Gas costs are reduced to absolute minimum.



Hyper/Normo Swings

High CO2 levels for a set period, then normal levels for a set period. Set the pattern to repeat as many times as needed. Any fluctuation can be created. Rapid swings, lengthy swings, increasing or decreasing durations; whatever CO2 model you need for your research, the OxyCycler A44C can handle it. oxycyclera44c08


Hypercapnia is a relative term that means excess concentration of CO2. The OxyCycler A44C can create hypercapnia by infusing the CO2 into the chamber until it reaches the setpoint and holding it.


Graded Hypercapnia

Raise CO2 levels gradually, or lower them gradually. The rise can be spread over days. Any rate of change can be set and repeated, faster or slower, and held for any length of time. oxycyclera44c09

Acute Hypercapnia

Sharp increase in CO2 levels can aid in psychological or neural models.

Works With Other Controllers

oxycyclera44c10The OxyCycler series was designed to be very flexible to meet researcher’s needs. This flexibility allows you to add additional gas controls down the line if you discover you need them. It also allows you to buy exactly what you need now, with confidence that you can add more later for new experiments. OxyCyclers can work together on the same chamber. Software configuration makes two controllers work together seamlessly as one. Each controls their respective gases simultaneously; even dynamic setpoints while holding other gases static.


Carbon Dioxide flux can affect physiology. It can only be studied if it can be recreated. Profiles are reproducible flux patterns. Profiles have multiple setpoints which change at precise times, with any rate of change from one setpoint to the next.


Carbon Dioxide profiles in a semi-sealed chamber are normobaric. Nitrogen and gas infusions displace chamber gas and equilibrates with ambient barometric pressure outside the chamber. Normobaric avoids hassles of pressure equipment. Chamber control avoids hassle of ventilation equipment.


Four chambers means one control profile and up to three experimental profiles. Comparing profiles makes optimizing models easy and straightforward. It also makes dose-response studies possible. Up to 17 different profiles can be stored. Each can be run or re-run in any of the four chambers at any time. Any given profile can be run in all the chambers simultaneously, or staggered at different times. Or every chamber can have a different profile running.


Pattern any carbon dioxide flux. Each profile can have 1- 20 set points. Straight line rate between any two sequential set points can be 0 – 999 minutes with resolution to seconds. Profiles can be cycled 1-99 times, or cycled continuously.