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OxyCycler A42OC

Dynamic O2 & CO2 Controller for Animal Modeling

Bioactive Gas Control System for in vivo:

  • Disease Modeling
  • Pathology Modeling
  • Therapy Modeling

with

  • Multiple chambers
  • Multiple set points
  • Multiple variables
When you publish your data generated with this equipment, please copy and paste as follows for proper citation in your manuscript.

OxyCycler A42OC Overview

MULTI-CHAMBER

Chambers can be controlled in many combinations. This includes simultaneous, independent gas control, different gas percentages in different chambers, or identical conditions at different times. You can independently control and profile two chambers at the same time, which means you have one control profile and one experimental profile.

MULTI-VARIABLE

O2 is a widely used single gas system for hypoxia and hyperoxia. CO2 is an animal off-gas that must be managed, but is also useful in modeling. N2 is biologically neutral, and thus used toflush chambers and lower either O2 or CO2 concentrations without interfering with the experiment. The OxyCycler A42OC manages the three simultaneously with a single controller.

CONTROL IS EFFICIENT

Feedback from the adapter pod’s sensors tells the OxyCycler A42OC the exact amount of gas to infuse. No gas is ever wasted with this O2/CO2 controller for animal modeling. Recovery after access is rapid within the BioSpherix sub chambers, which avoids prolonged deviations from the set-point.

MULTI-SETPOINT

The gas concentration can be programmed to change at any time. Simply program a series of setpoints each associated with a separate time. Intermittent hypoxia is a common example.

Dynamic control allows the operator the unique ability of simulating natural conditions, as well as creating and manipulating new artificial conditions.

OPERATION IS SIMPLE

Once the O2/CO2 controller for animal modeling is installed and configured, it’s easy. Check the calibration once in a while and don’t run out of gas. Otherwise it’s all automatic. The system can work continuously year round, or on occasion as needed.

 

ELECTRICAL POWER: 12 VDC @ 6.66A

CONTROL RANGE: 0.1-99.9% Oxygen, 0.1-20.0% Carbon Dioxide

ACCURACY: ± 1%

RESOLUTION: 0.1%

GAS SOURCE: Compressed gas tanks, liquid carboys (from headspace), or generators.

GAS SUPPLY: Nitrogen, Oxygen, Carbon Dioxide

GAS SUPPLY LINE: 1/4 inch I.D. hose pressure rated at 40 PSIG.

GAS SUPPLY LINE PRESSURE: 0-40 PSIG

GAS INFUSION RATE: 1-150 S.C.F.H. each control gas each chamber.

GAS SUPPLY HOSE FITTINGS: 1/4 inch hose barb.

UMBILICAL LENGTH: 12 feet (custom lengths available).

ACTUATOR POD SIZE: 7”h x 4.375”w x 4.5”d

ALARM OUTPUT: Visible flashing indicator. PC adds audible and more visible indicators.

ALARM MODES: Process high, process low, deviation high, deviation low, deviation band.

WEIGHT: 22lbs

DIMENSIONS: 9”h x 21”w x 18.5”d

HOST CHAMBER TEMPERATURE: 0-40°C

HOST CHAMBER CO2: 0.1%-20%

HOST CHAMBER HUMIDITY: 5-95% non-condensing

Sensor Operational Parameters

HOST CHAMBER TEMPERATURE: 0-40°C

HOST CHAMBER CO2: 0.1%-20%

HOST CHAMBER HUMIDITY: 5-95% non-condensing

Front Panel

oxycylera42oc12

1. Digital Display: Bright blue digits on black back ground. Continuously displays oxygen, carbon dioxide, control status, and alarm status in all chambers, unless pre-empted by touch-key operations. Displays menu items and settings during programming.

2. Bleed Barbs and Valves: Bleeds gases out of gas supply lines. Used for depressurizing gas supplies and manual sensor calibration.

3. ZERO Cal Gas Flowmeter: Used in calibration.

4. SPAN Cal Gas Flowmeter: Used in calibration.

5. Power Adjustments: Sets infusion rate of control gases in each chamber to accommodate different dynamics. Can manually override controller to shut off gas.

 

Back Panel

oxycylera42oc13

6. Power Receptacle: 12VDC power supply connects here.

7. Actuator Pod Umbilical: Flexible umbilicals connect remote actuator pods to back panel. Semi-swivel connectors at both ends allow 360° orientation. Some models are hard welded; function is the same.

8. Alarm Receptacle: Connect an appropriate alarm to this jack.

9. RS 485 Connections: One cable attaches to a computer and the other cable attaches to another unit, to allow communication with the computer (if applicable).

10. Accessory Jack: A port for accessories to attach to the controller

11. Supply Gas Hose Barb: Barbs for 1/4 inch I.D. hose from gas sources. Handles pressure up to 40 PSIG

12. Ground Stud: For grounding the unit to protect from electric damage.

 

Installation

oxycylera42oc031. Set OxyCycler A42OC on or near host chambers and plug it in.

2. Mount the adapter plate to its host chamber.

3. Hook up gas supply.

Operation

oxycylera42oc05Use PC software for easy interface, real-time trend charting, data logging, and remote operation.

The OxyCycler A42OC can be augmented with the OxyCycler AT Series controllers at any point to also grant control of CO/NO/NO2. The controllers work together to provide the most sophisticated exotic gas atmosphere control available.

This is illustrated in the image above. Note the unattached pod between the two chambers – this is a remote alarm monitoring pod that is part of the AT Series controllers.

This entire setup is generally placed within a BioSpherix Safety Hood (not pictured) to safely vent away any odors and exotic gas spill-off.

How It Works

oxycylera42oc04From outside two chambers, the OxyCycler A42OC works by remotely sensing oxygen and carbon dioxide inside each chamber, infusing nitrogen to reduce oxygen or carbon dioxide, and infusing oxygen or carbon dioxide to raise it. A continuous supply of both gases is required.

The OxyCycler A42OC connects to the chambers via two flexible umbilicals. At the tip of each umbilical is an actuator pod which contains a carbon dioxide sensor, oxygen sensor, a gas nozzle, and mounting hardware.

Pods mount to chamber over special precut holes so sensors can monitor chamber oxygen and carbon dioxide levels; gas can be infused when necessary.

Gas

USE ANY GAS SUPPLY

Conveniently utilizes gas from any source. Compressed gas is best in low consumption applications. Generator is best in high consumption applications. Liquid is best in between.

SAVES ON GAS

Maximum efficiency reduces chamber gas consumption

SAVES MONEY

Gas costs are reduced to absolute minimum.

 

oxycylera42oc06

Episodic Hypoxia

Recurring episodes of hypoxia can reproduce hypoxemia patterns created by occupational flight, sleep apnea, asthma, and pulmonary infections. Chronic hypoxic episodes might promote chronic degenerative diseases such as hypertension, diabetes, rheumatoid arthritis, macular degeneration, psoriasis, osteoporosis, etc.

oxycylera42oc07

Hyper/Hypo Swings

Hyperoxia for a day, then hypoxia for a day, back to hyperoxia for a day, hypoxia, hyperoxia, etc. quickly creates rich neovascularization in neonatal retina. Specifically models neovascular retinopathies. Nonspecifically models angiogenesis. Throws off redox regulation.

oxycylera42oc08

Acute Hyperoxia

Sudden increases in inspired oxygen can cause pulmonary damage. Model toxicity of oxygen therapy in young (premature born) and old (emphysemics) and in between (trauma patients). Model toxicity of recreational oxygen inhalation by athletes and revelers. Rate of oxygen increase can be adjusted faster or slower. Change faster to overwhelm antioxidants, or change slow to condition for antioxidants.

oxycylera42oc09

Graded Hypoxia

Model altitude acclimation. Or condition for hypoxia. Organs transplant better when prepared for the hypoxic journey. Conditioning can be gentle, but any rate of change can be set and repeated. Faster or slower, and held there any length of time.

oxycylera42oc10

Acute Intermittent Hypoxia

Hypoxia stress can model components of many severe diseases: heart attacks, stroke, asthma, choking, epilepsy, massive hemorrhage, etc. Deep sudden drops in chamber oxygen can create ischemia, apoptosis, and necrosis. Frequency, duration, and degree of drop are all adjustable.

■ O2  ■ CO2

Create Virtually any possible O2/CO2 model!

oxycylera42oc11

Works With Other Controllers

The OxyCycler series was designed to be very flexible to meet researcher’s needs. You never know exactly what you will discover when running animal experiments, and this flexibility allows you to add additional gas controls down the line if you discover you need them. OxyCyclers can work together on the same chamber. Software configuration makes two controllers work together seamlessly as one. Each controls their respective gases simultaneously; even dynamic setpoints while holding other gases static.

PROFILING IS FLUX

Oxygen and Carbon Dioxide flux can affect physiology. It can only be studied if it can be recreated. Profiles are reproducible flux patterns. Profiles have multiple set points which change at precise times, with any rate of change from one set point to the next.

NORMOBARIC FLUX

Oxygen and Carbon Dioxide profiles in a semi-sealed chamber are normobaric. O2, CO2 and N2 gas infusions displace chamber gas and equilibrates with the ambient barometric pressure outside. Normobaric avoids hassles of pressure equipment. Chamber control avoids hassle of ventilation equipment.

MULTIPLE PROFILING

Two chambers allow for an experimental protocol and a control protocol. Comparing profiles makes optimizing models easy and straightforward. It also makes dose-response studies possible. Up to 17 different profiles can be stored. Each can be run or re-run in any of the chambers at any time. Any given profile can be run in all the chambers simultaneously, or staggered at different times. Or every chamber can have a different profile running.

MODEL ANY OXYGEN OR CARBON DIOXIDE FLUX

Pattern any oxygen flux. Each profile can have 1- 20 set points. Set points can be anywhere from 0.1 – 99.9% oxygen and 0.1-20.0% carbon dioxide. Straight line rate between any two sequential set points can be 0 – 999 minutes with resolution to seconds. Profiles can be cycled 1-99 times, or looped to cycle continuously.