Cytocentric Blog

Top 5 Cytocentric Publications of 2017

With the first approvals of CAR-T, 2017 was a big year for cell-based research and therapies. Some scientific publications stood out from the rest as far as Cytocentric™ advancement. These publications not only encompass cutting-edge cell therapies, but also discoveries that were only made because researchers provided physiologically-relevant conditions for their cells in vitro.

 

Cytocentric Visionaries: Sausan Jaber and Evan Bordt

Do You Know? Oxygen and the Sex of Your Cultured Cells

Sausan Jaber and Evan Bordt are co-first-authors of a recent publication, “Sex differences in the mitochondrial bioenergetics of astrocytes but not microglia at a physiologically relevant brain oxygen tension”, in Neurochemistry International [1]. To control atmospheric conditions for their cells, they used an Xvivo System made by BioSpherix for research.

Sausan Jaber is working on a PhD at the University of Maryland, Baltimore and Evan Bordt recently started a post-doc at Massachusetts General Hospital and Harvard Medical School. Here, Dr. Alicia Henn interviews these young researchers about their recent paper. The conversation was edited for length.

Cytocentric Visionary: Jeffrey A. Stuart, Brock University

Oxygen: A Big Uncontrolled Variable in Cell Culture

Dr. Jeffrey A. Stuart is an Associate Professor of Biological Sciences at Brock University in St. Catherine’s, Canada. Dr. Alicia Henn interviews him here about his recent publication in BBRC, “Hydrogen peroxide production is affected by oxygen levels in mammalian cell culture. The transcript was edited for length.

Getting Started in Immunometabolism – Literature Resources for Innate Immunity

Innate Immunometabolism

Myeloid cells are subjected to highly variable environments in vivo and in vitro. Integrating environmental and cell-specific factors, the emerging field of Immunometabolism is making new connections between well-known metabolic mechanisms and specific innate immune functions. It is becoming increasingly clear that metabolic processes do more than just provide material support cell functions, but also contain key control points to innate immunity. Here are a few recent reviews and other resources to get you started in exploring Immunometabolism.

Getting Started in Immunometabolism – Literature Resources for Adaptive Immunity

Lymphocytes – An Army Marches on Its Metabolism

Lymphocytes migrate between wildly different microenvironments in vivo. Metabolic pathways, integrating cell-specific and site-specific factors, are intimately tied to activation, proliferation, and differentiation of lymphocytes. A circulating naïve lymphocyte may use Oxidative Phosphorylation (OxPhos) for energy, but it is non-proliferative. Blasting lymphocytes utilize glycolysis to meet high demands for metabolites for building biomass. Memory lymphocytes, often in low oxygen niches, favor glycolysis. Competition with other lymphocytes, tumor cells and microbes as well as the physiology of local nutrient and oxygen are environmental influences. Importantly, key control molecules in lymphocyte activation, such as mTOR, HIF, and NF-kappa B, are exquisitely sensitive to pericellular oxygen.