Cytocentric Blog

More Tips for Extreme Oxygen Studies:

Anoxia, Hypoxia, Physioxia and Hyperphysioxia

Cytocentric Basics: What oxygen level do the cells in my lab actually experience?

Researchers often shut the door on an incubator or chamber, check that the numbers on the outside for CO2 and O2 look good, and then turn away, assured that their cells are at the proper gas levels. However, setting up the atmosphere in any chamber does not mean that your cells inside experience that oxygen level.

Gases have to equilibrate with the liquid medium before they get to cells. Because oxygen does not dissolve well into aqueous solutions, if the medium is not pre-equilibrated to the desired oxygen levels, cells will experience unintended oxygen levels for a long time until the medium equilibrates with the chamber. See more information on that in a previous post.

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Cytocentric Visionaries: Lauren Zenewicz, University of Oklahoma Health Sciences Center

Don’t Miss Your Cells’ Phenotype by Using Inappropriate Room Air Oxygen

Dr. Lauren Zenewicz is an Assistant Professor in the Department of Microbiology and Immunology at the University of Oklahoma Health Sciences Center. Her lab studies Interleukin-22 and its role in the inflammation and disease. She published a recent article in Frontiers in Immunology on in vitro oxygen levels during immunological studies1. In this article, she proposes that oxygen be considered a microenvironmental trigger for immune cell signaling.

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Cytocentric Visionaries: Roger Rönn

The Cell Environment for HSC from iPS: Why Every Hurdle Must be Overcome to Reach our Destination

This is an update on a previous interview that Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with Roger Rönn, who recently finished his PhD and is now working in the laboratory of Elaine Dzierzak at the University of Edinburgh. Here we discuss his most recent paper in Stem Cells 1. This interview was edited for length.

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Cytocentric Visionaries: Romain Vuillefroy de Silly

Part Two: What’s in a Word? If It Describes the Oxygen Level, Quite a Bit.

Dr. Romain Vuillefroy de Silly is a post-doc at the Ludwig Center for Cancer Research of the UNIL in Lausanne, Switzerland. He has published original research papers and several extensive reviews on the role of oxygen in T cell function. This is Part Two of a Two-part interview that Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with him about his most recent review in Oncoimmunology. [1] This interview was edited for length.

Part One of our interview can be found here: Is Physiologic Oxygen be Better for CAR-T Cells In Vitro?

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Cytocentric Visionaries: Romain Vuillefroy de Silly

Part 1: Is Physiologic Oxygen Better for CAR-T Cells In Vitro?

Dr. Romain Vuillefroy de Silly is a post-doc at the University Ludwig center for Cancer Research of the UNIL in Lausanne, Switzerland. He has published original research and several compelling reviews on the role of oxygen in T cell function. Here is a written interview Dr. Alicia Henn, Chief Scientific Officer of BioSpherix, conducted with him about his most recent review in Oncoimmunology. [1] This interview was edited for length.

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