C-SHUTTLE (view literature)
Cell Culture GloveBox
O2/CO2 GLOVE BOX FOR HANDLING CELL CULTURES UNDER UNINTERRUPTED CONDITIONS
Hypoxia upregulated genes are immediately shut off by even brief exposures to room air.
The unique C-Shuttle Glove Box is a component of the SubChamber System. It provides a workspace for cell culture processing under the same O2/CO2 conditions that the cells live, while in the incubator.
Another component, the C-Chambers, provide a gas tight transport of cultures from the incubator to the workspace and back to the incubator
The pass through chamber, or antechamber, of the glove box is designed to fit C-Chambers. You can leave C-Chambers in the antechamber, and remove cells into uninterrupted O2/CO2 atmosphere, manipulate them without disturbance, and finally return them into the incubator with the same O2/CO2.
One C-Shuttle Glove Box can handle dozens of distributed C-Chambers around the building. You just have to take turns.
The C-Shuttle is made of white clean chemical resistant polypropylene. It is simple, practical and effective.
When working in a C-Shuttle your cells are never exposed to sub-optimal conditions. Whether you’re using Oxygen/CO2/Nitric Oxide your workspace and transport is always controlled to a precise and optimal level. Top scientists have been telling us for decades that the sudden shift of oxygen from open incubator doors and manipulating cells in normal room air is causing havoc on HIF and other transcription factors.
5% of the Genome is regulated by oxygen.
Clinical evidence is mounting regarding oxygen's role in gene expression. Difference in culture and processing conditions lead to inconsistent results. Differences in culture and processing conditions leads to inconsistent results.
In open labs with open hoods, cells and researchers share the critical workspace most of the time. The majority of contamination in labs is caused by humans and their bioburden. With a closed environment provided with the C-Shuttle and C-Chambers the main source of contamination is removed.
C-Shuttles protect the researchers
Cells are often purposely grown and processed to generate or test viruses and vectors. More worrisome is when cells are unknowingly infected with viruses or other transmissible agents. C-Chambers will limit exposure to harmful viruses, vectors and prions where conventional open labs allow the technicians to breathe potentially dangerous non-cellular biological entities.
Softside gloves are most comfortable and flexible. Iris ports are also an option. Two sets of gloves allow two different people to access the workspace at the same time.