The Lasker Awards were won this year by a trio of incredibly important figures in physiologic oxygen research; William Kaelin, Peter Ratcliffe, and Gregg Semenza.
What did they do?
An important new publication in Stem Cells and Development by Tiwari et al in Australia has brought a critically important in vitro oxygen diffusion issue to the forefront. In a murine model, they explore optimum oxygen levels for best in vitro expansion of HSC for transplant.
In a closed system like a barrier isolator, there are few routes for disinfectants to leave and no way for contaminants to float in.
In an open laboratory, you can spray surfaces liberally with disinfectant and it all evaporates into the room air. Keeping surfaces wet with disinfectant for maximum contact time is the major concern.
It is a big change, moving from an open lab, which exists in a constant state of high microbial contamination risks, into the closed Xvivo system, a very low risk environment. A risk-based balance has to be struck between much lower risk of contamination and the higher risk of disinfectant fume build-up.
In Part Two, we discussed Trailhead’s unbiased system of cell signaling optimization for differentiation. Today, Dr. Jan Jensen. CSO of Trailhead BioSystems discusses full-time optimization of cell culture conditions with us.
In Part One, we discussed Trailhead’s unbiased system of cell signaling optimization for differentiation. Here we continue our conversation with Dr. Jan Jensen. CSO of Trailhead BioSystems in Cleveland, OH. Today we talk about cell fate and reprogramming.